不同Zeta电位纳米脂蛋白载体的物理稳定性评价  

Physical stability evaluation of nanolipoprotein carriers with different Zeta potentials

作　　者：宋璇 秦迎丹 孙晨 刘勃 孙玉琦 SONG Xuan;QIN Yingdan;SUN Chen;LIU Bo;SUN Yuqi(College of Pharmacy,Jinzhou Medical University,Liaoning Jinzhou 121000,China)

机构地区：[1]锦州医科大学药学院,辽宁锦州121000

出　　处：《中国医院药学杂志》2023年第12期1342-1346,共5页Chinese Journal of Hospital Pharmacy

基　　金：辽宁省教育厅基本科研面上项目(编号:LJKZ0804)。

摘　　要：目的:模拟天然脂蛋白结构,构建不同Zeta电位的纳米脂蛋白载体,考察纳米脂蛋白载体电位与物理稳定性之间的关系。方法:以十六烷基三甲基溴化铵、聚山梨酯80、牛磺胆酸钠为乳化剂,采用乳化-溶剂蒸发法制备不同Zeta电位纳米脂蛋白载体,考察体外释放性能。评价其贮藏稳定性、蛋白结合稳定性与膜稳定性等物理稳定性,对电位与稳定性进行关联分析。结果:制备的3种纳米脂蛋白载体粒径大小接近,Zeta电位分别为24.77 mV、-11.50 mV和-28.90 mV,荷正电的纳米脂蛋白载体释放平稳,且贮藏稳定性较好,蛋白结合率高,半数纳米脂蛋白载体膜被破坏所需曲拉通X-100的浓度为5.94%,膜稳定性较好。荷负电的纳米脂蛋白载体物理稳定性较差。结论:不同乳化剂能够用于不同Zeta电位纳米脂蛋白载体的制备,电位与载体的物理稳定性具有一定关联,荷正电的纳米脂蛋白载体物理稳定性较荷负电的好。OBJECTIVE To construct nanolipoprotein carriers with different Zeta potentials by simulating the structure of natural lipoprotein and investigate the relationship between potentials and physical stability of nanolipoproteins carriers.METHODS In emulsified solvent evaporation method,the nanolipoprotein carriers with different Zeta potentials were prepared,using cetyl trimethyl ammonium bromide,tween 80 and sodium taurocholate as emulsifiers,and the release performance was investigated in vitro.The storage stability,protein binding stability and membrane stability were evaluated.The correlation between potentials and physical stability was analyzed.RESULTS The particle sizes for three kinds of nanolipoprotein carriers were similar.But the Zeta potentials were different with 24.77 mV,-11.50 mV or-28.90 mV.Positive charged nanolipoprotein carrier release smoothly,and the storage stability was good,the protein binding proportion was high.The concentration of Triton X-100 needed to destroy half of the nanolipoprotein carrier membrane was 5.94%,and the membrane stability was good.The negative charged nanolipoprotein carriers had poor physical stability.CONCLUSION Different emulsifiers can be used for the preparation of nanolipoprotein carriers with different Zeta potentials,and the potential is related to the physical stability of the carrier.The physical stability of positive charged nanolipoprotein carrier is better than that of negative charged nanolipoprotein carrier.

关 键 词：纳米脂蛋白载体 ZETA电位 贮藏稳定性 蛋白结合稳定性 膜稳定性 

分 类 号：R943[医药卫生—药剂学]