松墨天牛肠道BS16基因组及其纤维素酶活特性分析  

Genome Analysis of Monochamus alternatus Gut BS16 and Characterization of Its Cellulase Activity

作　　者：李国强 罗巧玉 杨梅娇 初旭 马秋雨 张飞萍[1,2] 胡霞 Li Guoqiang;Luo Qiaoyu;Yang Mejiao;Chu Xu;Ma Qiuyu;hang Feiping;Hu Xi(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou Fujian 350002,China;Key Laboratory of Integrated Pest Management in Ecological Forests,Fuzhou Fujian 350002,China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]生态公益林重大有害生物防控福建省高校重点实验室,福建福州350002

出　　处：《西南林业大学学报（自然科学）》2023年第4期100-108,共9页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家自然基金青年项目(31800548)资助;国家重点研发计划项目(2017YFD0600105)资助;福建省林业科学项目[闽林科(2019)16号]资助。

摘　　要：蜡状芽孢杆菌BS16是从松墨天牛肠道筛选获得的1株具有纤维素酶活性的细菌,为了解BS16的菌株基因组特性及其纤维素降解功能,采用扫描电镜、基因组学分析、DNS法探究BS16的形态特征、纤维素酶解基因、纤维素酶活。结果表明:BS16的细胞为胶囊状,长为3~4μm,直径为0.5~1.2μm;蜡状芽孢杆菌BS16基因组全长5 347 914 bp,包含5 494个基因,其中包括5 338个蛋白质编码基因、11个rRNA基因、102个t RNA基因和17个sRNA基因;BS16基因组中主要由GH4、GH5、GH8、GH13家族编码纤维素酶;蜡状芽孢杆菌BS16具有完整的纤维素酶系,在其分泌的纤维素酶中内切葡聚糖酶活性最高为(2.186±0.081)U/mL,其内切葡聚糖酶的酶活性最适pH值为5.0、最适温度为40℃。Bacillus cereus BS16 is a bacterium with cellulase activity screened from the intestinal tract of Monochamus alternatus.In order to understand the genomic characteristics of the BS16 strain and its cellulose degradation function,this study used scanning electron microscopy,genomics analysis,and DNS method to explore the morphological characteristics,cellulase enzymatic gene,and cellulase activity of BS16.The results showed that the cells of BS16 were capsules with a length of 3-4μm and a diameter of 0.5-1.2μm.The full length of B cereus BS16 genome is 5347914 bp,containing 5494 genes,including 5338 protein-coding genes,11 rRNA genes,102 tRNA genes and 17 sRNA genes.Cellulase is mainly encoded by GH4,GH5,GH8 and GH13 families in BS16 genome.B.cereus BS16 has a complete cellulase system and the highest endoglucanase activity was 2.186±0.081 U/mL.The optimal pH value and temperature of endoglucanase activity are 5.0 and 40℃respectively.

关 键 词：蜡状芽孢杆菌 基因组 纤维素酶 内切葡聚糖酶 

分 类 号：S763.38[农业科学—森林保护学]