大熊猫源纤维素分解菌的筛选及产酶发酵条件优化研究  

Screening of Cellulolytic Bacteria from Giant Panda and Optimization of Enzyme-producing Fermentation Conditions

作　　者：田思齐 郭爱伟 和芳 彭晓武 李有龙 杨亚晋 Tian Siqi;Guo Aiwei;He Fang;Peng Xiaowu;Li Youlong;Yang Yajin(College of Life Sciences,Southwest Forestry University,Kunming Yunnan 650233,China;Yunnan Wild Animal Park Co.Ltd.,Kunming Yunnan 650233,China)

机构地区：[1]西南林业大学生命科学学院,云南昆明650233 [2]云南野生动物园有限公司,云南昆明650233

出　　处：《西南林业大学学报（自然科学）》2023年第4期197-204,共8页Journal of Southwest Forestry University：Natural Sciences

基　　金：云南省教育厅科学研究基金项目(2022J0504)资助;西南林业大学教育科学研究项目(QN202103)资助;生物学质量工程项目(503190106)资助;国家自然科学基金项目(31860650)资助。

摘　　要：通过采集昆明野生动物园里大熊猫新鲜粪便,利用羧甲基纤维素钠培养基(CMC-Na)进行选择性培养,刚果红平皿浸染初筛和DNS法纤维素酶活复筛,对目标菌株进行形态学和16S rDNA同源性鉴定,并对高产纤维素酶的菌株进行单因素发酵条件优化试验。结果表明:从大熊猫新鲜粪便中筛选到2株高效的纤维素分解菌鉴定为枯草芽孢杆菌ZD1和MZ3(ZD1/MZ3),2个菌株发酵液纤维素酶活分别为277.58U/mL和255.22U/mL,通过发酵条件优化后,ZD1和MZ3发酵液中纤维素酶最大活力分别达339.48U/mL和293.65U/mL,较优化前酶活可提高22.30%和15.06%,最佳产酶条件为:CMC-Na+P、37℃、pH6.8~7.2、36~48h。Through collecting the fresh faeces of giant panda in Kunming Wild Animal Park,after preliminary screening of using with carboxymethylcellulose sodium(CMC-Na)and Congo red staining method to selective culture,and then determining of cellulase activity by DNS method for rescreening,the target strain was identified by morphology and 16SrDNA homology,and the strain with high cellulase production was optimized by single factor fermentation optimization.The results showed that 2 strains of celluloytic bacterium were identified as Bacillus subtilis ZD1 and MZ3 from fresh faeces of giant panda,the activity of cellulase in ZD1 and MZ3 is 277.58 U/mL and 255.22 U/mL after the fermentation optimization,the maximal activity of cellulase in ZD1 and MZ3 is up to 339.48 U/mL and 293.65 U/mL respectively,which is 22.30% and 15.06% higher than that before the optimization.The selected strains were very active as follow:using medium of CMC-Na+P,culture temperature 37℃,PH value of 6.8 to 7.2,culture time 36 hours to 48 hours.

关 键 词：大熊猫 纤维素分解菌 枯草芽孢杆菌 纤维素酶 发酵优化 

分 类 号：S718.65[农业科学—林学]