The Sm core protein SmEb regulates salt stress responses through maintaining proper splicing of RCD1 pre-mRNA in Arabidopsis  

作　　者：Yechun Hong Yang Gao Jia Pang Huazhong Shi Tingting Li Huiying Meng Dali Kong Yunjuan Chen Jian-Kang Zhu Zhen Wang 

机构地区：[1]Institute of Advanced Biotechnology and School of Life Sciences,Southern University of Science and Technology,Shenzhen 518055,China [2]Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China [3]School of Life Sciences,Anhui Agricultural University,Hefei 230036,China [4]Department of Chemistry and Biochemistry,Texas Tech University,Lubbock,Texas 79409,USA

出　　处：《Journal of Integrative Plant Biology》2023年第6期1383-1393,共11页植物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32000206 to Z.W.and 32200234 to Y.H.);Anhui Provincial Natural Science Foundation(2208085Y08 to Z.W.);Shenzhen Science and Technology Program(RCBS20210706092213009 to Y.H.)。

摘　　要：Salt stress adversely impacts crop production.Several spliceosome components have been implicated in regulating salt stress responses in plants,however,the underlying molecular basis is still unclear.Here we report that the spliceosomal core protein SmEb is essential to salt tolerance in Arabidopsis.Transcriptome analysis showed that SmEb modulates alternative splicing of hundreds of pre-mRNAs in plant response to salt stress.Further study revealed that SmEb is crucial in maintaining proper ratio of two RCD1 splicing variants(RCD1.1/RCD1.2)important for salt stress response.In addition,RCD1.1 but not RCD1.2 is able to interact with the stress regulators and attenuates saltsensitivity by decreasing salt-induced cell death in smeb-1 mutant.Together,our findings uncovered the essential role of SmEb in the regulation of alternative pre-mRNA splicing in salt stress response.

关 键 词：alternative splicing RCD1 salt stress SmEb SPLICEOSOME 

分 类 号：Q945[生物学—植物学]