High-quality Fagopyrum esculentum genome provides insights into the flavonoid accumulation among different tissues and self-incompatibility  

作　　者：Qiang He Dan Ma Wei Li Longsheng Xing Hongyu Zhang Yu Wang Cailian Du Xuanzhao Li Zheng Jia Xiuxiu Li Jianan Liu Ze Liu Yuqing Miao Rui Feng Yang Lv Meijia Wang Hongwei Lu Xiaochen Li Yao Xiao Ruyu Wang Hanfei Liang Qinghong Zhou Lijun Zhang Chengzhi Liang Huilong Du 

机构地区：[1]School of Life Sciences,Institute of Life Sciences and Green Development,Hebei University,Baoding 071000,China [2]State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,Innovative Academy for Seed Design,Chinese Academy of Sciences,Beijing 100101,China [3]State Key Laboratory of Rice Biology,China National Rice Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 310000,China [4]Center for Agricultural Genetic Resources Research,Shanxi Agricultural University,Taiyuan 030031,China [5]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Integrative Plant Biology》2023年第6期1423-1441,共19页植物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32100500);Natural Science Foundation of Hebei Province(C2021201048);supported by the fund from the State Key Laboratory of Plant Genomics and the Chinese Academy of Sciences(CASWX2021SF-0109)。

摘　　要：Common buckwheat(Fagopyrum esculentum)and Tartary buckwheat(Fagopyrum tataricum),the two most widely cultivated buckwheat species,differ greatly in flavonoid content and reproductive mode.Here,we report the first high-quality and chromosome-level genome assembly of common buckwheat with 1.2 Gb.Comparative genomic analysis revealed that common buckwheat underwent a burst of long terminal repeat retrotransposons insertion accompanied by numerous large chromosome rearrangements after divergence from Tartary buckwheat.Moreover,multiple gene families involved in stress tolerance and flavonoid biosynthesis such as multidrug and toxic compound extrusion(MATE)and chalcone synthase(CHS)underwent significant expansion in buckwheat,especially in common buckwheat.Integrated multi-omics analysis identified high expression of catechin biosynthesis-related genes in flower and seed in common buckwheat and high expression of rutin biosynthesis-related genes in seed in Tartary buckwheat as being important for the differences in flavonoid type and content between these buckwheat species.We also identified a candidate key rutindegrading enzyme gene(Ft8.2377)that was highly expressed in Tartary buckwheat seed.In addition,we identified a haplotype-resolved candidate locus containing many genes reportedly associated with the development of flower and pollen,which was potentially related to self-incompatibility in common buckwheat.Our study provides important resources facilitating future functional genomics-related research of flavonoid biosynthesis and selfincompatibility in buckwheat.

关 键 词：BUCKWHEAT comparative genomics flavonoid biosynthesis genome evolution SELF-INCOMPATIBILITY 

分 类 号：S517[农业科学—作物学]