九味化斑丸化学成分及其治疗银屑病的作用机制分析  

Chemical components and mechanism of Jiuwei Huaban pill in treating psoriasis

作　　者：隋银海 郝磊 霍志鹏 王玉[2,3,4] 何毅[2,3] SUI Yin‐hai;HAO Lei;HUO Zhi‐peng;WANG Yu;HE Yi(Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Development Center of Modern Chinese Medicine,Research Institute of Tasly Holding Group Co.Ltd.,Tianjin 300410,China;State Key Laboratory of Critical Technology in Innovative Chinese Medicine,Tasly Pharmaceutical Group Co.Ltd.,Tianjin 300410,China;School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072,China)

机构地区：[1]天津中医药大学,天津301617 [2]天士力控股集团有限公司研究院现代中药开发中心,天津300410 [3]天士力医药集团股份有限公司创新中药关键技术国家重点实验室,天津300410 [4]天津大学药物科学与技术学院,天津300072

出　　处：《海南医学院学报》2023年第14期1082-1092,共11页Journal of Hainan Medical University

基　　金：国家“重大新药创制”科技重大专项(2017ZX09301005)。

摘　　要：目的:系统研究九味化斑丸治疗银屑病的关键成分并对其作用机制进行探讨。方法:基于UPLC‐Q‐TOF/MSE对九味化斑丸的化学成分进行全面分析与鉴定,利用SwissADME平台和TCMSP数据库筛选活性成分,采用网络药理学的方法研究九味化斑丸治疗银屑病的作用机制,采用分子对接技术验证关键成分与靶点蛋白的结合活性。结果:从九味化斑丸中共鉴定出75个成分,其中治疗银屑病的活性成分有木犀草素、黄芩素、黄芩苷、芍药苷和栀子苷等35个。30个核心靶点富集分析结果显示九味化斑丸主要通过调节IL‐17信号通路、TNF信号通路、PI3K‐Akt信号通路、Th17信号通路、MAPK信号通路发挥治疗银屑病的作用。“成分‐靶点‐通路”网络中度值排名靠前的活性成分及靶点有较强的结合活性。结论:初步阐明了九味化斑丸治疗银屑病的有效成分和作用机制,为质量控制提供参考依据。Objective:To systematically study the key active ingredients of Jiuwei Huaban pill in the treatment of psoriasis and explore its mechanism of action.Methods:The chemical components of Jiuwei Huaban pill were systematically identified by UPLC‐QTOF/MSE,and network pharmacology method was used to study the main pharmacodynamic substances of Jiuwei Huaban pill and discuss the mechanism of action.Molecular docking technology was used to verify the binding activity of key components and target proteins.Results:A total of 75 components of Jiuwei Huaban pill were identified,35 of which were key components for psoriasis treatment,including luteolin,baicalin,baicalin,paeoniflorin and geniposide.Enrichment analysis of 30 core target pathways showed that Jiuwei Huaban pill played its role in the treatment of psoriasis from the IL‐17 signaling pathway,TNF signaling pathway,PI3K‐Akt signaling pathway,Th17 signaling pathway,and MAPK signaling pathway.The key active components in Jiuwei Huaban pill and targets with the highest DGREE value in the"component target disease"network have strong binding activity.Conclusion:The active ingredients and mechanism of action of Jiuwei Huaban pill in the treatment of psoriasis were preliminarily clarified,which provids reference for quality control.

关 键 词：九味化斑丸 银屑病 网络药理学 分子对接 UPLC‐Q‐TOF/MSE 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]