睡眠剥夺对豚鼠离焦性近视及视网膜多巴胺代谢的影响  

Contributions of Sleep Deprivation to the Development of Lens-Induced Myopia and Retinal Dopamine Metabolism in Guinea Pigs

作　　者：杨雪莉 陈尧[1] 关宇欣 袁超群 毛俊峰[1] Xueli Yang;Yao Chen;Yuxin Guan;Chaoqun Yuan;Junfeng Mao(Ophthalmology Center,Xiangya Hospital,Central South University,Hunan Provincial Key Laboratory of Ophthalmology,Changsha 410008,China)

机构地区：[1]中南大学湘雅医院眼科中心眼科学湖南省重点实验室,长沙410008

出　　处：《中华眼视光学与视觉科学杂志》2023年第7期499-505,共7页Chinese Journal Of Optometry Ophthalmology And Visual Science

基　　金：湖南省自然科学基金项目(2021JJ31088)。

摘　　要：目的:探究睡眠剥夺(SD)对豚鼠光学离焦性近视(LIM)形成及视网膜近视相关信号因子多巴胺(DA)代谢的影响。方法:实验研究。2022年3月采用随机数表法将30只21日龄三色豚鼠分为LIM组和LIM+SD组,予以右眼离焦处理,左眼为自身对照,所有实验动物予以14 d正常睡眠环境或每天SD处理20 h。每组进行角膜曲率半径、屈光度和眼轴长度等生物学参数测量。采用高效液相色谱电化学法测定视网膜DA、3,4-二羟基苯乙酸(DOPAC)含量,计算DOPAC/DA比值;通过免疫组织化学染色法及Western blot检测视网膜酪氨酸羟化酶(TH)分布及表达。豚鼠左右眼屈光参数及神经递质含量比较采用配对样本t检验,LIM组与LIM+SD组间屈光参数及神经递质含量比较采用独立样本t检验。离焦眼视网膜DA含量分别与眼球屈光度、眼轴长度进行Pearson相关分析。结果:LIM组对照眼与LIM+SD组对照眼屈光度、眼轴长度比较差异均无统计学意义(均P>0.05);与LIM组离焦眼相比,LIM+SD组离焦眼的近视程度更深、眼轴延长更长(t=5.63,P<0.001;t=-3.87,P=0.001)。2组豚鼠离焦眼视网膜DA、DOPAC含量均低于自身对照眼(LIM组:t=-8.64,P<0.001;t=-13.03,P<0.001;LIM+SD组:t=-11.72,P<0.001;t=-16.32,P<0.001),其中LIM+SD组离焦眼视网膜DA、DOPAC含量均较LIM组更低(t=5.35,P<0.001;t=3.80,P=0.002),而DOPAC/DA比值升高且组间差异具有统计学意义(t=-3.60,P=0.003)。豚鼠离焦眼视网膜DA含量与屈光度、眼轴长度均有相关性(r=0.93,P<0.001;r=-0.77,P=0.001)。免疫组织化学染色结果显示,TH阳性反应细胞主要分布在视网膜神经纤维层、神经节细胞层、内丛状层和内核层,外丛状层可见少量着色;与自身对照眼相比,豚鼠离焦眼视网膜染色均减弱。Western blot检测显示,离焦眼视网膜TH蛋白表达均明显低于自身对照眼(LIM组:t=-40.25,P<0.001;LIM+SD组:t=-17.64,P<0.001),但LIM组与LIM+SD组离焦眼视网膜TH蛋白表达差异无统计�Objective:To investigate the effects of sleep deprivation(SD)on the development of lens-induced myopia(LIM)and the dopamine(DA)metabolism in guinea pigs.Methods:This was an animal experimental study.A total of 30 three-week-old guinea pigs supplied were randomly divided into two groups:LIM group and LIM+SD group,raised either in a normal sleep environment or subjected to sleep deprivation for 20 hours every day.Refraction,corneal curvature radius,and ocular axial components were measured.The content of DA and 3,4-dihydroxyphenylacetic acid(DOPAC)in the retina was assessed by the highperformance liquid chromatography electrochemical method,and the DOPAC/DA ratio was calculated.The expression of retinal tyrosine hydroxylase(TH)protein was evaluated by immunohistochemical staining and Western blot.Paired t-test was used to compare the refraction and DA turnover between the left and right eyes of guinea pigs.Independent sample t-test was used for comparison between the LIM group and the LIM+SD group.The refractive error and axial length of the guinea pig eyes in the defocus eyes underwent a correlation analysis with DA content by using Pearson correlation analysis.Results:Normal refractive development was not affected by SD in guinea pigs.However,SD significantly deepened myopia and prolonged the ocular axis of defocus eyes(t=5.63,P<0.001;t=-3.87,P=0.001).The content of retinal DA and DOPAC in all defocus eyes was lower than that in their control eyes(LIM group:t=-8.64,P<0.001;t=-13.03,P<0.001;LIM+SD group:t=-11.72,P<0.001;t=-16.32,P<0.001).Compared with LIM group,the defocus eyes of LIM+SD group had lower DA and DOPAC obviously(t=5.35,P<0.001;t=3.80,P=0.002),while the DOPAC/DA ratio increased and the difference was statistically significant(t=-3.60,P=0.003).The content of retinal DA in defocus eyes was correlated with refractive error(r=0.93,P<0.001)and eye axis length(r=-0.77,P=0.001).Retinal immunohistochemical staining showed that TH positive cells were mainly distributed in the nerve fiber layer,ganglion cell la

关 键 词：睡眠剥夺 光学离焦性近视眼 视网膜 多巴胺 酪氨酸羟化酶 

分 类 号：R778.11[医药卫生—眼科]