肾细胞癌组织中lncRNA CRNDE、miR-29a-3p表达及临床意义  

The expression and clinical significance of lncRNA CRNDE and miR-29a-3p in renal cell carcinoma tissue

作　　者：王晨宇[1] 郭峰[1] 赵建华[1] 罗勇[1] Wang Chenyu;Guo Feng;Zhao Jianhua;Luo Yong(Urology Surgery,Xinjiang Uygur Autonomous Region People's Hospital,Xinjiang Province,Urumqi 830001,China)

机构地区：[1]新疆维吾尔自治区人民医院泌尿外科,乌鲁木齐830001

出　　处：《疑难病杂志》2023年第7期702-707,718,共7页Chinese Journal of Difficult and Complicated Cases

基　　金：新疆维吾尔自治区自然科学基金资助项目(2022D01C615)。

摘　　要：目的分析肾细胞癌(RCC)组织中长链非编码RNA结直肠肿瘤差异表达基因(lncRNA CRNDE)、微小RNA-29a-3p(miR-29a-3p)表达与临床病理特征和预后的关系。方法选取2018年1月—2019年11月新疆维吾尔自治区人民医院泌尿外科收治RCC患者92例,qPCR检测RCC组织和癌旁组织中lncRNA CRNDE、miR-29a-3p表达,分析二者的相关性及与临床病理特征的关系。采用K-M法绘制不同lncRNA CRNDE、miR-29a-3p表达RCC患者生存曲线,采用多因素Cox回归分析RCC患者死亡的影响因素。结果与癌旁组织比较,RCC癌组织中lncRNA CRNDE表达升高,miR-29a-3p表达降低(t=16.050、21.147,P均<0.001)。Pearson相关性分析显示,RCC癌组织中lncRNA CRNDE与miR-29a-3p表达呈负相关(r/P=-0.739/<0.001)。RCC癌组织中lncRNA CRNDE在WHO/ISUP分级3~4级、TNM分期Ⅲ~Ⅳ期、有淋巴结转移中高于WHO/ISUP分级1~2级、TNM分期Ⅰ~Ⅱ期、无淋巴结转移者(t/P=3.041/0.003、3.183/0.002、3.598/0.001),而miR-29a-3p表达低于WHO/ISUP分级1~2级、TNM分期Ⅰ~Ⅱ期、无淋巴结转移(t/P=3.197/0.002、2.962/0.004、3.421/0.001)。随访3年,92例RCC患者累积生存率为81.52%(75/92)。K-M生存曲线分析显示,lncRNA CRNDE高表达组累积生存率低于lncRNA CRNDE低表达组,miR-29a-3p高表达组累积生存率高于miR-29a-3p低表达组(χ^(2)/P=4.346/0.037、5.773/0.016)。多因Cox回归分析显示,WHO/ISUP分级3~4级、TNM分期Ⅲ~Ⅳ期、淋巴结转移、lncRNA CRNDE高为RCC患者死亡的独立危险因素,miR-29a-3p高为独立保护因素[HR(95%CI)=4.112(1.676~10.088)、4.664(1.696~12.825)、5.806(1.881~17.919)、1.552(1.144~2.105)、0.524(0.312~0.883)]。结论RCC组织中lncRNA CRNDE高表达和miR-29a-3p低表达,与WHO/ISUP分级、TNM分期、淋巴结转移和预后有关,可能成为RCC诊治的新靶点。Objective To analyze the relationship between the expression of differentially expressed genes(lncRNA CRNDE)and microRNA-29a-3p(miR-29a-3p)in colorectal tumors with long chain non coding RNA in renal cell carcinoma(RCC)tissue,clinical pathological features,and prognosis.Methods From January 2018 to November 2019,92 patients with RCC were selected from the Urology of the People's Hospital of Xinjiang.qPCR was used to detect the expression of lncRNA CRNDE and miR-29a-3p in RCC tissues and paracancerous tissues,and analyze their correlation and relationship with clinicopathological characteristics.The survival curves of RCC patients with different lncRNA CRNDE and miR-29a-3p expressions were plotted using the K-M method,and the influencing factors of mortality in RCC patients were analyzed using multivariate Cox regression.Results Compared with adjacent tissues,the expression of lncRNA and CRNDE in RCC cancer tissue increased,while the expression of miR-29a-3p decreased(t=16.050,21.147,P<0.001).Pearson correlation analysis showed a negative correlation between lncRNA CRNDE and miR-29a-3p expression in RCC cancer tissue(r/P=-0.739/<0.001).In RCC cancer tissue,lncRNA CRNDE expression was higher in WHO/ISUP grades 3-4,TNM stages III-IV,and with lymph node metastasis than in WHO/ISUP grades 1-2,TNM stages I-II,and without lymph node metastasis(t/P=3.041/0.003,3.183/0.002,3.598/0.001),while miR-29a-3p expression was lower in WHO/ISUP grades 1-2,TNM stages I-II,and without lymph node metastasis(t/P=3.197/0.002,2.962/0.004,3.421/0.001).Following up for 3 years,the cumulative survival rate of 92 RCC patients was 81.52%(75/92).K-M survival curve analysis showed that the cumulative survival rate of the lncRNA CRNDE high expression group was lower than that of the lncRNA CRNDE low expression group,and the cumulative survival rate of the miR-29a-3p high expression group was higher than that of the miR-29a-3p low expression group(χ^(2)/P=4.346/0.037,5.773/0.016).Multivariate Cox regression analysis showed that WHO/ISUP grade 3-

关 键 词：肾细胞癌 长链非编码RNA 结直肠肿瘤差异表达基因 微小RNA-29a-3p 临床病理特征 预后 

分 类 号：R737.11[医药卫生—肿瘤]