不同碘水平下血清外泌体miRNA的表达对甲状腺乳头状癌淋巴结转移的早期诊断价值  

The early diagnostic value of serum exosome miRNA expression on lymph node metastasis of papillary thyroid carcinoma at different iodine levels

作　　者：刘新悦 赵鸿渐 邢浩[3] 尹峰燕[3] 刘庆华 孔伟 何倩 邢薇佳 LIU Xinyue;ZHAO Hongjian;XING Hao;YIN Fengyan;LIU Qinghua;KONG Wei;HE Qian;XING Weijia(School of Public Health and Health Management,Shandong First Medical University,Shandong Jinan 250000,China;Department ofGeneral Surgery,Shandong Province Chengwu Hospital,Shandong Heze 274200,China;Department of Thyroid Surgery,Taian CentralHospital,Shandong Taian 271000,China;Department of Clinical Laboratory,the Second AffiliatedHospital of Shandong First Medical University,Shandong Taian 271000,China;Department of Thyroid Surgery,the Second AffiliatedHospital of Shandong First Medical University,Shandong Taian 271000,China)

机构地区：[1]山东第一医科大学公共卫生与健康管理学院,山东济南250000 [2]山东省成武县人民医院普外科,山东菏泽274200 [3]泰安市中心医院甲状腺外科,山东泰安271000 [4]山东第一医科大学第二附属医院检验医学科,山东泰安271000 [5]山东第一医科大学第二附属医院甲状腺外科,山东泰安271000

出　　处：《现代肿瘤医学》2023年第16期3014-3018,共5页Journal of Modern Oncology

基　　金：山东省医药卫生科技发展计划项目(编号:202012050653)。

摘　　要：目的:探讨甲状腺乳头状癌(papillary thyroid carcinoma,PTC)淋巴结转移(lymph node metastasis,LNM)患者在高碘及正常碘水平下的血清外泌体微小RNA(microRNA,miRNA)差异表达谱,为不同碘水平下PTC LNM的早期鉴别诊断提供参考依据。方法:选取高碘及正常碘PTC LNM^(+)组与PTC LNM-组各5例样本,提取血清外泌体miRNA进行高通量测序,筛选不同碘水平下PTC LNM^(+)组共有的差异表达miRNA。并进行靶基因预测、GO富集分析和KEGG富集分析。结果:高碘和正常碘PTC LNM^(+)组共有的差异表达外泌体miRNA有5个,其中hsa-miR-7113-5p、hsa-miR-873-3p、hsa-miR-548q在不同碘水平下表达情况相反,hsa-miR-616-3p、hsa-miR-5189-5p均表达上调。差异外泌体miRNA调控HIF-1信号通路、神经活性配体-受体的相互作用和GnRH信号通路。结论:血清外泌体hsa-miR-7113-5p、hsa-miR-873-3p、hsa-miR-548q用于筛选PTC LNM时,应区分高碘及正常碘水平;hsa-miR-616-3p、hsa-miR-5189-5p可用于一般人群PTC LNM的早期诊断。Objective:To explore the differential expression profile of serum exosome microRNA(miRNA)in patients with papillary thyroid carcinoma(PTC)lymph node metastasis(LNM)under high iodine and normal iodine levels,and to provide reference for the early differential diagnosis of PTC LNM at different iodine levels.Methods:Five patients in the high iodine and normal iodine PTC LNM^(+)group and PTC LNM-group were selected for high-throughput sequencing of serum exosome miRNA.The common differential expression of exosome miRNA in PTC LNM^(+)group at different iodine levels was screened.The target gene prediction,GO and KEGG enrichment analysis were carried out.Results:There were five kinds of differential expression of exosome miRNAs in the high iodine and normal iodine PTC LNM^(+)groups.The expression of hsa-miR-7113-5p,hsa-miR-873-3p and hsa-miR-548q was opposite at different iodine levels,and the expression of hsa-miR-616-3p and hsa-miR-5189-5p was up-regulated.The differentially expressed exosome miRNA regulated HIF-1 signaling pathway,neuroactive ligand-receptor interaction and GnRH signaling pathway.Conclusion:When serum exosome hsa-miR-7113-5p,hsa-miR-873-3p and hsa-miR-548q are used to screen PTC LNM,high iodine and normal iodine levels should be distinguished.The hsa-miR-616-3p and hsa-miR-5189-5p can be used for the early diagnosis of PTC LNM in the general population.

关 键 词：甲状腺乳头状癌 淋巴结转移 碘 外泌体 MICRORNA 

分 类 号：R739.91[医药卫生—肿瘤]