APLN及其受体在肝细胞癌中的表达及其临床意义  

The expression and clinical significance of APLN and its receptor in hepatocellular carcinoma

作　　者：朱东来 王婷 陆静 葛国洪 邵江波 ZHU Donglai;WANG Ting;LU Jing;GE Guohon;SHAO Jiangbo(Department of Laboratory Medicine,The Third People′s Hospital of Zhenjiang City,Zhenjiang,Jiangsu 212000,China;Internal Medicine,The Third People′s Hospital of Zhenjiang City,Zhenjiang,Jiangsu 212000,China;Department of Hepatology,The Third People′s Hospital of Zhenjiang City,Zhenjiang,Jiangsu 212000,China)

机构地区：[1]镇江市第三人民医院检验科,江苏镇江212000 [2]镇江市第三人民医院内科,江苏镇江212000 [3]镇江市第三人民医院肝病科,江苏镇江212000

出　　处：《现代医药卫生》2023年第14期2400-2406,共7页Journal of Modern Medicine & Health

基　　金：江苏省镇江市社会发展基金项目(FZ2020049)。

摘　　要：目的阐明肝细胞癌(HCC)爱帕琳肽(APLN)和APLN受体(APLNR)的表达及其影响HCC发生、发展的潜在机制。方法使用UALCAN、GEPIA网站对HCC APLN和APLNR表达及其对生存率的影响进行分析,再使用TIMER网站和STRING数据库对APLN促进HCC发生、发展的可能分子机制进行分析。结果与正常样本比较,HCC APLN转录水平明显上调,APLNR转录水平无明显变化。APLN高表达的HCC患者生存期明显缩短,APLNR表达水平与HCC患者生存预后无明显相关性。异常表达的APLN分子与血管内皮生长因子A(VEGFA)呈明显正相关,低氧诱导因子1、Fms相关酪氨酸激酶1、神经纤毛蛋白2、VEGFR2均与APLN-VEGFA存在相互作用,且主要参与调节内皮细胞增殖和血管形成等。结论APLN在HCC中明显高表达且不利于患者生存预后,APLN可能通过调节VEGFA促进肿瘤血管内皮细胞增殖和血管形成等机制,促进HCC的发生、发展,提示APLN是HCC新的潜在诊断标志物和治疗靶点。Objective To elucidate the expression of Apelin(APLN)and APLN receptor(APLNR)in hepatocellular carcinoma(HCC)and its potential mechanism affecting the occurrence and development of HCC.Methods UALCAN and GEPIA websites were used to analyze the expression of APLN and APLNR in HCC and their effects on survival rate.Then TIMER website and STRING database were used to analyze the possible molecular mechanism of APLN promoting the occurrence and development of HCC.Results Compared with normal samples,the transcription level of APLN in HCC was significantly up-regulated,and the transcription level of APLNR was not significantly changed.The survival of HCC patients with high APLN expression was significantly shortened,and there was no significant correlation between APLNR expression level and survival prognosis of HCC patients.The abnormal expression of APLN was positively correlated with vascular endothelial growth factor A(VEGFA).Hypoxia inducer factor 1,FMS-related tyrosine kinase 1,neurociliin 2 and VEGFR2 all interact with APLN-VEGFA,and were mainly involved in regulating endothelial cell proliferation and angiogenesis.Conclusion APLN is significantly highly expressed in HCC and is not conducive to the survival and prognosis of patients.APLN may promote the occurrence and development of HCC by regulating VEGFA to promote the proliferation and angiogenesis of tumor vascular endothelial cells,suggesting that APLN is a new potential diagnostic marker and therapeutic target for HCC.[Key words]

关 键 词：肝细胞癌 爱帕琳肽 APLN受体 预后 生物信息学分析 

分 类 号：R735.7[医药卫生—肿瘤]