siRNA沉默水通道蛋白5基因抑制人肝癌细胞的生长作用  

Silencing aquaporin 5 gene by siRNA inhibits the growth of human hepatoma cells

作　　者：郭陶陶 韩艳珍[2] 单铁强[3] 单铁英[4] 聂晓进[5] 刘记平 GUO Tao-tao;HAN Yan-zhen;SHAN Tie-qiang;SHAN Tie-ying;NIE Xiao-jin;LIU Ji-ping(Department of laboratory medicine,Handan second hospital,Handan 056002,Hebei;Department of nursing,Affiliate hospital of Hebei Engineering University,Handan 056002,Hebei;Department of laboratory medicine,Qinhuangdao harbor hospital,Qinhuangdao 066000,Hebei;Medical college,Hebei University of Engineering,Handan 056002,Hebei;Department of thoracic surgery,Handan Central Hospital,Handan 056002,Hebei;Handan Vocational College of science and technology,Handan 056002,Hebei,China)

机构地区：[1]邯郸市第二医院检验科,056002 [2]河北工程大学附属医院护理,056002 [3]河北省秦皇岛市海港医院检验科,066000 [4]河北工程大学医学院,056002 [5]邯郸市中心医院胸外科,056002 [6]邯郸科技职业学院,056002

出　　处：《现代消化及介入诊疗》2023年第4期427-429,436,共4页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：河北省卫健委立项课题(20220653)。

摘　　要：目的通过采用小干扰RNA(siRNA)靶向作用人肝癌细胞Bel-7402中的水通道蛋白5(AQP5)基因,观察其对Bel-7402的增殖及凋亡的作用。方法体外孵育Bel-7402,采用阴性对照siRNA(siRNA-NC)、siRNA-AQP5#1、siRNA-AQP5#2转染细胞。实验设置细胞为对照组、siRNA-NC组、sliRNA-AQP5#1组、siRNA-AQP5#2组。噻唑蓝染色检测细胞的增殖能力;流式细胞技术测定细胞的凋亡情况;蛋白免疫印迹技术和Real time-PCR测量细胞中AQP5蛋白和mRNA的含量。结果siRNA-NC组与对照组相比,细胞的增殖、凋亡及AQP5蛋白和mRNA的含量无明显差异(P>0.05)。与对照组相比,siRNA-AQP5#1组、siRNA-AQP5#2组的细胞的增殖力降低、凋亡率升高、AQP5蛋白和mRNA的含量均降低(均P<0.05)。结论siRNA靶向作用人肝癌细胞AQP5基因通过降低其增殖和升高其凋亡率达到抑制癌细胞的生长作用。Objective The small interfering RNA(siRNA)targeting the aquaporin 5(AQP5)gene in human hepatoma cell Bel-7402 was used,we observe its effects on the proliferation and apoptosis of Bel-7402.Methods Bel-7402 was incubated in vitro and transfected with negative control siRNA(siRNA-NC),siRNA-AQP5#1 and siRNA-AQP5#2.The cells were set as control group,siRNA NC group,siRNA-AQP5#1 group and siRNA-AQP5#2 group.The proliferation ability of cells was detected by thiazole blue staining;The apoptosis of cells was measured by flow cytometry;The contents of AQP5 protein and mRNA in cells were measured by Western blot and real time PCR.Results The cell proliferation,apoptosis,AQP5 protein and mRNA had not obvious difference between siRNA-NC group and control group(P>0.05).Marched with the control group,the proliferation of cells decreased,the apoptosis rate increased,and the contents of AQP5 protein and mRNA decreased in siRNA-AQP5#1 group and siRNA-AQP5#2 group(all P<0.05).Conclusion siRNA targeting AQP5 gene can inhibit the growth of human hepatoma cells by reducing their proliferation and increasing their apoptosis rate.

关 键 词：小干扰RNA 水通道蛋白5 人肝癌细胞 增殖 凋亡 

分 类 号：R575[医药卫生—消化系统]