组蛋白去甲基化酶KDM8促进卵巢癌细胞增殖和转移  

Histone lysine demethylases 8 promotes proliferation and metastasis of ovarian cancer cells

作　　者：郑乐朋[1] 刘春艳[1] 卢文波[1] 刘文渊[1] 苏鑫怡 李卓玲 ZHENG Le-peng;LIU Chun-yan;LU Wen-bo;LIU Wen-yuan;SU Xin-yi;LI Zhuo-ling(Department of Laboratory,Ningbo Women and Children's Hospital,Zhejiang 315012,China)

机构地区：[1]宁波市妇女儿童医院检验科,浙江315012

出　　处：《中国卫生检验杂志》2023年第12期1413-1417,共5页Chinese Journal of Health Laboratory Technology

基　　金：宁波市自然科学基金(202003N4287);宁波市妇女保健学重点学科(2022-F27);宁波市医学科技计划项目(2019Y17)。

摘　　要：目的探讨卵巢癌细胞中组蛋白去甲基化酶KDM8对细胞增殖和转移的作用及其机制。方法采取Western blot检测卵巢癌组织和癌旁组织中KDM8和己糖激酶HK2蛋白质表达水平;CCK8、克隆形成、流式细胞术、细胞划痕和Transwell侵袭实验检测沉默KDM8后细胞增殖、凋亡和侵袭水平;葡萄糖测试盒和乳酸测试盒检测沉默KDM8后细胞有氧糖酵解水平。结果卵巢癌组织中KDM8和HK2蛋白质水平高于癌旁组织(P<0.01);与对照组相比,沉默KDM8后卵巢癌细胞的增殖能力降低(P<0.001),克隆形成数减少(P<0.05),细胞凋亡比例增加(P<0.001),G1期细胞比例增加(P<0.001),细胞迁移和侵袭能力下降(P<0.001),葡萄糖摄取率和乳酸生成减少(P<0.001)。结论KDM8可能通过调控HK2介导的有氧糖酵解促进卵巢癌细胞的增殖和转移。Objective This paper aims to investigate the effect of histone lysine demethylases 8(KDM8)on proliferation and metastasis of ovarian cancer cells.Methods The protein expressions of KDM8 and hexokinase 2(HK2)in ovarian cancer tissues and adjacent tissues were detected by Western blot.Cell counting kit-8(CCK-8),colony formation assay,flow cytometry,wound-healing assay and Transwell cell invasion assay were used to detect the effects of RNA interference of KDM8 expression on proliferation,apoptosis and invasion.Results The protein levels of KDM8 and HK2 in ovarian cancer tissues were higher than those in adjacent tissues(P<0.01).Compared with the control group,after silting KDM8,the proliferation ability of ovarian cancer cells decreased(P<0.001),the number of clones decreased(P<0.05),the proportion of apoptosis increased(P<0.001),the proportion of G1 cells increased(P<0.001),and the cell migration and invasion ability decreased(P<0.001).Glucose uptake rate and lactic acid production were decreased(P<0.001).Conclusion KDM8 could promote the proliferation and metastasis of ovarian cancer cells by regulating the expression of HK2.

关 键 词：卵巢癌 KDM8 HK2 有氧糖酵解 

分 类 号：R737.31[医药卫生—肿瘤]