单宁酸对感染猪流行性腹泻病毒仔猪生长性能、肠道屏障功能和抗氧化能力的影响  

Effects of Tannic Acid on Growth Performance,Intestinal Barrier Function and Antioxidant Capacity of Piglets Infected with Porcine Epidemic Diarrhea Virus

作　　者：王思甜 徐朋涛 郑丽云 齐雅 刘志鹏 何来 刘诚傲 侯永清[1] 郭双双[1] 童庆芳 张正帆 丁斌鹰[1] WANG Sitian;XU Pengtao;ZHENG Liyun;QI Ya;LIU Zhipeng;HE Lai;LIU Cheng’ao;HOU Yongqing;GUO Shuangshuang;TONG Qingfang;ZHANG Zhengfan;DING Binying(Engineering Research Center of Feed Protein Resources on Agricultural By-Products,Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University,Wuhan 430023,China;Wuhan Jiangxia District Animal Disease Prevention and Control Center,Wuhan 430072,China)

机构地区：[1]武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,动物营养与饲料科学湖北省重点实验室,武汉430023 [2]武汉市江夏区动物疫病预防控制中心,武汉430072

出　　处：《动物营养学报》2023年第7期4276-4286,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：湖北省中央引导地方科技发展专项(2021BGE047);湖北省重点攻关项目(2022BBA0014)。

摘　　要：本研究旨在探讨单宁酸(TA)对感染猪流行性腹泻病毒(PEDV)仔猪生长性能、肠道屏障功能和抗氧化能力的影响。试验选用24头初始体重为(2.72±0.22)kg的健康7日龄杜×长×大仔猪,随机分为4组,分别为对照组、TA组、PEDV组和PEDV+TA组,每组6个重复,每个重复1头猪。试验期为12 d,分为感染前(第1~8天)和感染后(第9~12天)2个阶段。在第1~8天,TA组和PEDV+TA组仔猪口腔灌服190 mg/kg BW TA,对照组和PEDV组仔猪口腔灌服相同体积的液体代乳料;在第9天,PEDV组和PEDV+TA组仔猪口腔灌服1 mL 106TCID_(50)/头PEDV悬液(TCID_(50)表示50%组织培养感染剂量),对照组和TA组仔猪灌服等体积的磷酸盐缓冲溶液。结果显示:感染PEDV前,TA组仔猪平均日增重(ADG)较对照组显著升高(P<0.05)。感染PEDV后,仔猪ADG显著降低(P<0.05),料重比(F/G)和腹泻指数显著升高(P<0.05);血清D-木糖含量显著下降(P<0.05),血清二胺氧化酶活性与十二指肠、空肠、回肠和结肠肠道损伤评分显著升高(P<0.05);十二指肠、空肠和回肠绒毛高度(VH)与十二指肠和回肠绒毛面积(VA)显著降低(P<0.05),空肠、回肠和结肠隐窝深度(CD)显著增加(P<0.05),十二指肠、空肠和回肠VH/CD显著降低(P<0.05);血清谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、总超氧化物歧化酶(T-SOD)活性显著降低(P<0.05),十二指肠、空肠、结肠GSH-Px活性与回肠、结肠T-SOD活性以及空肠总抗氧化能力(T-AOC)显著下降(P<0.05),空肠丙二醛(MDA)含量与十二指肠和结肠过氧化氢(H_(2)O_(2))含量显著增加(P<0.05)。灌服TA显著缓解了PEDV感染造成的仔猪F/G、腹泻指数、血清谷丙转氨酶(ALT)活性以及十二指肠、空肠、回肠和结肠CD增加(P<0.05),显著缓解了PEDV感染造成的仔猪ADG、空肠VH、十二指肠和空肠VA和VH/CD下降(P<0.05),并显著提高了PEDV感染仔猪十二指肠和空肠GSH-Px活性与结肠T-SOD活性以及十二指肠和结肠T-AOC(P<0.05)。�This study was conducted to investigate the effects of tannic acid(TA)on the growth performance,intestinal barrier function and antioxidant capacity of piglets infected with porcine epidemic diarrhea virus(PEDV).Twenty⁃four 7⁃day⁃old healthy piglets(Duroc×Landrace×Yorkshire),with an initial body weight of(2.72±0.22)kg,were divided into 4 groups with 6 replicates per group,and 1 pig per replicate.The groups were as follows:control group,TA group,PEDV group and PEDV+TA group.The experiment was divided into 2 stages as pre⁃infected stage(days 1 to 8)and infected stage(days 9 to 12).On days 1 to 8,piglets in the TA group and PEDV+TA group were orally administered with 190 mg/kg BW TA,and piglets in the con⁃trol group and PEDV group were orally administered with liquid milk replacer at the same volume;on day 9,piglets in the PEDV group and PEDV+TA group were orally administered PEDV suspension at a dose of 106 TCID_(50)(50%tissue culture infection dose)per pig,piglets in the control group and TA group were orally administered with phosphate buffered solution at the same volume.The results showed that,before PEDV in⁃fection,the average daily gain(ADG)of piglets in TA group was significantly increased compared with the control group(P<0.05).After PEDV infection,the ADG of piglets was significantly decreased(P<0.05),and the feed/gain(F/G)and diarrhea index were significantly increased(P<0.05);the serum D⁃xylose con⁃tent was significantly decreased(P<0.05),and serum diamine oxidase activity and intestinal damage scores of duodenum,jejunum,ileum and colon were significantly increased(P<0.05);the villus height(VH)of duo⁃denum,jejunum and ileum and the villus area(VA)of duodenum and ileum were significantly decreased(P<0.05),the crypt depth(CD)of jejunum,ileum and colon was significantly increased(P<0.05),and the VH/CD of duodenum,jejunum and ileum was significantly decreased(P<0.05);the activities of glutathione peroxidase(GSH⁃Px),catalase(CAT)and total superoxide dismutase(T⁃SOD)in serum were signi

关 键 词：单宁酸 猪流行性腹泻病毒 生长性能 肠道屏障功能 抗氧化能力 

分 类 号：S828[农业科学—畜牧学]