Genome-Wide Analysis of the F3′5′H Gene Family in Blueberry(Vaccinium corymbosum L.)Provides Insights into the Regulation of Anthocyanin Biosynthesis  

作　　者：Xiaolan Guo Jinbin Hu Shimei Yang Delu Wang Jianbing Wang 

机构地区：[1]College of Life Sciences,Huizhou University,Huizhou,516007,China [2]College of Forestry,Guizhou University,Guiyang,550025,China [3]School of Architecture and Civil Engineering,Huizhou University,Huizhou,516007,China [4]Guizhou Institute of Subtropical Crop.,Guizhou Academy of Agricultural Sciences,Xingyi,562400,China

出　　处：《Phyton-International Journal of Experimental Botany》2023年第9期2683-2697,共15页国际实验植物学杂志（英文）

基　　金：funded by the National Natural Science Foundation of China(Grant No.31760205);Guangdong Basic and Applied Basic Research Foundation of Guangdong Province-Yuehui Joint Foundation(Grant No.2022A1515111095);the Professor and Doctoral Research Project(Grant No.2022JB021)in Huizhou University;the Project of the Department of Education of Guangdong Province(Grant No.2018KTSCX214).

摘　　要：The F3′5′H gene family plays an important role in regulating anthocyanin biosynthesis,abiotic stress,and hormone signaling.In this study,14 F3′5′H genes were identified from the blueberry genome.The chromosomal distribution,physicochemical properties,F3′5′H domain,conserved motifs,cis-acting elements,and intron/exon compositions were analyzed.The functional prediction analysis of these VcF3′5′Hs indicated that their biological functions included light response and other secondary metabolites.The results of qRT-PCR showed that VcF3′5′Hs(especially VcF3′5′H4)were highly expressed at the ripening stage.Subcellular localization revealed that VcF3′5′H4 may be located in the endoplasmic reticulum.Co-expression analysis showed that the VcF3′5′H gene family was related to anthocyanin.This research provides an overview of the blueberry F3′5′H family and helps verify the role of these genes in regulating anthocyanin biosynthesis.

关 键 词：BLUEBERRY F3′5′H ANTHOCYANIN gene expression CO-EXPRESSION 

分 类 号：Q94[生物学—植物学]