EFFECTS OF TRANSPORT STRESS ON THE INTESTINES INVOLVING NEURONAL NITRIC OXIDE SYNTHASE  

作　　者：Jing LAN Tonghui MA Peng YIN Kedao TENG Yunfei MA 

机构地区：[1]College of Veterinary Medicine,China Agricultural University,Beijing 100193,China [2]School of Engineering Medicine,Beihang University,Beijing 100191,China

出　　处：《Frontiers of Agricultural Science and Engineering》2023年第2期285-295,共11页农业科学与工程前沿（英文版）

基　　金：supported by the National Natural Science Foundation of China(31772686 and 31502025);the National TwelveFive Technological Supported Plan of China(2011BAD34B01)。

摘　　要：Transport stress is commonly suffered by animals with gastrointestinal dysfunction a common symptom.Currently,the mechanisms of transport stress-induced intestine impairment are largely unknown.The aim of this study was to investigate the effects of transport stress on the expression of neuronal nitric oxide synthase(nNOS)and the distribution of nNOS-positive neurons of the intestines in rats and to explore the neuroendocrine mechanism of transport stress.In this study,Sprague Dawley rats(n=6)were subjected on a constant temperature shaker for 1(S1d)or 3 d(S3d).Rats exhibited increased serum glucose and diminished total number of leukocytes,in which lymphocytes level was also decreased in the S1d group(P<0.05).Also,normal intestinal morphology was disrupted in the S1d rats,and the thickness of muscle layers was decreased in duodenum,jejunum and colon of S3d rats.In addition,it was found that nNOS expression,as well as the number of nNOSpositive neurons in the myenteric plexus were downregulated in duodenum,jejunum and colon of S3d rats compared with that of unstressed rats(P<0.05).These data reveals that transport stress induced intestinal damage and uncovers potential action mechanisms that nNOS-positive neurons and nNOS expression might be involved in modulating this process.

关 键 词：INTESTINE NNOS nNOS-positive neurons transport stress 

分 类 号：S852.2[农业科学—基础兽医学]