黄腐酸对口腔表皮样癌KB细胞的抗肿瘤效应及光热作用  

Anti-tumor effect of fulvic acid on oral epidermoid carcinoma KB cells and its photothermal role

作　　者：王士睿 辛鹏飞 张彩凤 刘青梅 WANG Shirui;XIN Pengfei;ZHANG Caifeng;LIU Qingmei(Department of Stomatology,School and Hospital of Stomatology,Shanxi Medical University,Taiyuan 030001,China;Department of Stomatology,Third Hospital of Shanxi Medical University,Shanxi Bethune Hospital,Shanxi Academy of Medical Sciences,Tongji Shanxi Hospital;Department of Chemistry,Taiyuan Normal University,Humic Acid Engineering Technology Research Center of Shanxi Province)

机构地区：[1]山西医科大学口腔医学院口腔医院口腔科,太原030001 [2]山西医科大学第三医院,山西白求恩医院,山西医学科学院,同济山西医院口腔科 [3]太原师范学院化学系,山西省腐植酸工程技术研究中心

出　　处：《山西医科大学学报》2023年第6期741-746,共6页Journal of Shanxi Medical University

基　　金：量子光学与光量子器件国家重点实验室(山西大学)开放课题(KF202213);山西省基础研究计划项目(202203021221240);山西白求恩医院“烽火计划”人才培养基金项目(2022FH18)。

摘　　要：目的探究黄腐酸(fulvic acid,FA)的体外光热升温性能,及对口腔表皮样癌KB细胞的增殖作用,并研究FA联合808 nm激光对KB细胞的光热治疗效果。方法使用808 nm激光照射FA,红外热像仪记录温度变化。将FA与口腔表皮样癌KB细胞共培养,按0.25,0.50,1.00,2.00,3.00 mg/ml的FA浓度加入含KB细胞的培养基,以未加FA处理为对照,通过CCK-8实验检测其对细胞的毒性。将FA联合808 nm激光照射KB细胞,分为:0.50 W/cm^(2)激光+1.00 mg/ml FA组,1.00 W/cm^(2)激光+0.50 mg/ml FA组和2.00 W/cm^(2)激光+0.25 mg/ml FA组。三组分别以不同功率密度激光照射不同FA浓度的含KB细胞的培养基10 min;分别以未加FA处理单纯使用各自功率密度的激光照射10 min为相应对照组。通过CCK-8实验及活死细胞染色实验检测其对细胞的光热治疗作用。结果不同浓度FA在808 nm激光照射下温度升高,并逐渐达到峰值。将0.25,0.50,1.00,2.00,3.00 mg/ml的FA与KB细胞共培养24 h后,细胞毒性检测结果显示,细胞活力有明显下降,并呈剂量依赖性(P<0.05)。光热治疗结果显示,将0.25,0.50,1.00 mg/ml的FA与KB细胞共培养24 h后,分别使用不同功率密度激光照射10 min,与各自对照组比较,FA联合激光照射组细胞存活率显著降低(P<0.0001)。FA联合激光照射KB细胞后,经活死细胞染色示视野内可见大量发出红色荧光的死细胞,仅见少量绿色标记的活细胞。结论FA具有优异的光热转换性能;FA可以抑制口腔表皮样癌KB细胞的增殖;FA结合低能量激光能产热,有效杀灭口腔表皮样癌KB细胞。Objective To explore the in vitro photothermal heating performance of fulvic acid(FA)and its effect on the proliferation of oral epidermal carcinoma KB cells,and investigate the photothermal therapeutic effect of FA combined with laser irradiation at 808 nm on KB cells.Methods FA was irradiated by laser at 808 nm and the corresponding temperature changes were recorded via a thermal imager.After 0.25,0.50,1.00,2.00,3.00 mg/ml FA were respectively added into the culture media containing oral epidermoid carcinoma KB cells,its cytotoxicity was detected by CCK-8,with FA-free treatment as the corresponding control groups.The KB cells treated with FA and laser irradiation at 808 nm were further divided into three groups:0.50 W/cm^(2) laser+1.00 mg/ml FA group,1.00 W/cm^(2) laser+0.50 mg/ml FA group,and 2.00 W/cm^(2) laser+0.25 mg/ml FA group.The culture media containing KB cells in the three groups were treated with FA at different concentrations and irradiated with lasers at different power densities for 10 min,and the cells treated with irradiation at different power densities for 10 min alone but no FA treatment were established as the corresponding control groups,respectively.The photothermal therapeutic effect of FA on KB cells was examined by CCK-8 and live-dead cell staining.Results The temperature of FA with different concentrations increased under laser irradiation at 808 nm and gradually reached its peak.After co-culture of KB cells with FA at 0.25,0.50,1.00,2.00,3.00 mg/ml for 24 h,the results of the cytotoxicity test showed a significant decrease in cell viability in a dose-dependent manner(P<0.05).The results of photothermal therapy indicated that the cell survival rates were significantly lower in laser+FA groups than those in the corresponding groups(P<0.0001).After KB cells were treated by FA combined with laser irradiation,the live-dead cell staining revealed a large number of dead cells with red fluorescence,and only a small number of green fluorescence-labeled live cells.Conclusion FA has excellent ph

关 键 词：黄腐酸 口腔表皮样癌 光热治疗 光热剂 低能量激光 

分 类 号：R739.8[医药卫生—肿瘤]