EZH2基因在宫颈癌顺铂耐药中的作用及机制  

Effect of EZH2 gene on cisplatin resistance in cervical cancer and its mechanism

作　　者：陈茜 王海燕[1] 刘宪 刘佳 CHEN Qian;WANG Haiyan;LIU Xian;LIU Jia(Department of Reproductive Medicine,the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,Shaanxi,China;Department of Thoracic Surgery,Tumor Hospital of Shaanxi province,Xi'an 710061,Shaanxi,China)

机构地区：[1]西安交通大学第一附属医院生殖医学科,陕西西安710061 [2]陕西省肿瘤医院胸外科,陕西西安710061

出　　处：《贵州医科大学学报》2023年第7期774-779,786,共7页Journal of Guizhou Medical University

基　　金：国家自然科学基金〔2017〕81702579;陕西省肿瘤医院院基金(SC211004)。

摘　　要：目的探讨EZH2在宫颈癌中对顺铂耐药的作用及机制。方法将宫颈癌细胞分为对照组+干扰EZH2(shRNA)组、对照组+过表达EZH2组、对照组+GSK343处理组及对照组+顺铂(DDP)耐药组,采用MTT法检测细胞耐药性,实时荧光定量PCR检测EZH2、ABCG2 mRNA表达水平,蛋白印迹法检测EZH2、ABCG2蛋白表达情况。结果与对照组比较,干扰EZH2组顺铂IC50降低,细胞抑制率升高,ABCG2 mRNA和蛋白表达降低;过表达EZH2组顺铂IC50升高,细胞抑制率降低,ABCG2 mRNA和蛋白表达升高;GSK343处理组顺铂IC50降低,细胞抑制率升高;与对照组比较,顺铂耐药组EZH2、ABCG2的mRNA和蛋白表达升高,差异均有统计学意义(P<0.05)。结论EZH2诱导人宫颈癌细胞对顺铂的耐药,其机制可能与调控细胞耐药相关基因ABCG2的表达有关。Objective To investigate the role and regulatory mechanism of EZH2 gene in cisplatin resistance in cervical cancer.Methods Cervical cancer cells were divided into the control group+interference EZH2(shRNA)group,the control group+overexpression EZH2 group,the control group+GSK343 treatment group and the control group+cisplatin(DDP)resistance group.The drug resistance of cells was detected by MTT method.The mRNA expression levels of EZH2 and ABCG2 were detected by real-time fluorescence quantitative PCR,and the protein expression of EZH2 and ABCG2 were detected by protein printing.Results Compared with the control group,the cisplatin IC50 decreased,the cell inhibition rate increased,and the expression of ABCG2 mRNA and protein decreased in the EZH2 interference group.In EZH2 overexpression group,cisplatin IC50 increased,the cell inhibition rate decreased,and the expression of ABCG2 mRNA and protein increased.Cisplatin IC50 decreased and cell inhibition rate increased in GSK343 treatment group.Compared with the control group,the mRNA and protein expressions of EZH2 and ABCG2 in cisplatin resistance group were significantly higher,and the differences were statistically significant(P<0.05).Conclusion EZH2 can induce cisplatin resistance in human cervical cancer cells,and its mechanism may be related to the regulation of the expression of drug resistance related gene ABCG2.

关 键 词：宫颈肿瘤 基因 EZH2 顺铂 耐药性 蛋白表达 

分 类 号：R737.3[医药卫生—肿瘤]