Assessment of d ifferent f ormalin c oncentrations on the h istochemical s taining of l iver,k idneys and l ungs of Wistar r ats  

作　　者：Gideon Taiwo Oluwaloye Ochuko Fortunate Jenakevwe Kenneth Chukwudi Onyegbula Julius Kayode Bankole Seyi Samson Enitan 

机构地区：[1]Department of Medical Laboratory Science,Babcock University,Ilisan-Remo,121103,Ogun State,Nigeria [2]Department of Biomedical Laboratory Science,University of Ibadan,Ibadan,200005,Oyo State,Nigeria [3]Department of Medical Laboratory Science,Ambrose Alli University,Ekpoma,310006,Edo State,Nigeria

出　　处：《Toxicology Advances》2023年第3期22-27,共6页毒理学前沿

摘　　要：Background:Formaldehyde is a chemical substance used to preserve the biochemical and physiological components of a tissue by preventing autolysis and putrefaction,leaving the tissue in a life-like manner.Objective:This study aims to assess the effect of formalin concentrations on the histochemical staining in liver,kidneys and lungs of Wistar rats.Methods:A total of ten(10)Wistar rats were purchased and allowed to acclimatize for a period of 2 weeks in plastic cages with iron netting at the Animal House facility,Babcock University.The animals were placed on standard rat feeds and water ad-libitum.The rats were randomly sacrificed by cervical dislocation,following mild anaesthesia using chloroform inhalation.For histological examination,the liver,lungs,and kidneys of Wistar rats were removed and preserved in neutral buffered formalin at 2%,4%,6%,8%,and 10%concentrations.Sections were stained in Haematoxylin and Eosin(Vector Laboratories,Inc.Newark,US).The quality of the nuclei,cytoplasm and cell membrane were graded on a scale of 1(poor)to 5(excellent).Results:Six percent(6%)formalin was observed to produce excellent results in liver tissues with a total grade of sixteen(16).10%formalin produced excellent results in lung tissues with a total grade or nineteen(19).Interestingly,2%,6%and 10%formalin concentrations produced excellently results in kidney tissues with the highest total grade fourteen(14).Conclusion:This study concludes that 6%formalin produced better section and staining qualities more than the commonly used 10%formalin according to the observations from the stained sections.This implies that the concentration of formalin affects the histochemical staining in liver,kidneys and lungs of Wistar rats.

关 键 词：FIXATION KIDNEY liver lungs neutral buffered formalin Wistar rats 

分 类 号：X70[环境科学与工程—环境工程]