肉桂油和八角茴香油的体内外抗菌作用研究  

Study on antibacterial activity of Cinnamon oil and star anise oil in vitro and in vivo

作　　者：易丹丹 覃兰迁 刘霞 刘宇[1] 吴莉芩 相亦飞 莫奕豪 何家康[1] YI Dan-dan;QIN Lan-qian;LIU Xia;LIU Yu;WU Li-qin;XIANG Yi-fei;MO Yi-hao;HE Jia-kang

机构地区：[1]广西大学动物科学技术学院,广西南宁530005

出　　处：《饲料研究》2023年第13期60-64,共5页Feed Research

基　　金：国家自然科学基金项目(项目编号:31960717)。

摘　　要：试验旨在研究肉桂油和八角茴香油的体内外抗菌效果。通过微量稀释法、棋盘法、稀释涂布平板法分别测定肉桂油和八角茴香油对大肠杆菌和沙门氏菌的最小抑菌浓度(MIC)、联合抑菌作用及抑菌曲线。采用大肠杆菌致小鼠腹泻病理模型验证肉桂油和八角茴香油对小鼠的保护作用。结果显示,肉桂油对大肠杆菌和沙门氏菌的MIC分别为0.31~0.62 mg/L、0.19~0.39 mg/L。八角茴香油对大肠杆菌和沙门氏菌的MIC均在50~100 mg/L。联合抑菌结果显示,肉桂油和八角茴香油联用对大肠杆菌和沙门氏菌的抑菌浓度指数(FIC)值分别为0.16~0.53、0.16~0.28。抑菌曲线结果显示,肉桂油和八角茴香油单用及联用均能够抑制细菌生长,且联用后抑菌效果增强。动物试验结果显示,小鼠血清中肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)含量与模型组相比显著下降。研究表明,肉桂油和八角茴香油单用或联用对大肠杆菌和沙门氏菌具有抑制作用,对大肠杆菌引起的小鼠腹泻具有一定的保护作用。The experiment was to study antibacterial effect of Cinnamon oil and star anise oil in vivo and in vitro.The minimum inhibitory concentration(MIC),combined inhibitory effect and inhibitory curve of Cinnamon oil and star anise oil against Escherichia coli and Salmonella were determined by microdilution method,chessboard method and dilution coating plate method respectively.The protective effect of Cinnamon oil and star anise oil on mice diarrhea induced by Escherichia coli were further verified.The results showed that MIC of Cinnamon oil against Escherichia coli and Salmonella was 0.31~0.62 mg/L and 0.19~0.39 mg/L,MIC of star anise oil against Escherichia coli and Salmonella was 50~100 mg/L.The results of combined inhibitory effect showed that FIC value of combination of Cinnamon oil and star anise oil was 0.16-0.53 for Escherichia coli and 0.16~0.28 for Salmonella.The results of inhibitory curve showed that both single and combined use of Cinnamon oil and star anise oil can inhibit growth of Bacteria,and inhibitory effect was increased after combined use.The animal test results showed that compared with model group,the content of TNF-αand IL-1βin mouse serum were significantly decreased.The experiment indicates that Cinnamon oil and star anise oil alone or combination can inhibition effect on Escherichia coli and Salmonella,and have certain protective effect on mice diarrhea caused byEscherichiacoli.

关 键 词：肉桂油 八角茴香油 抑菌作用 大肠杆菌 沙门氏菌 

分 类 号：S816.7[农业科学—饲料科学]