血小板源性微粒在口腔鳞癌中的表达及临床意义  

Expression and Clinical Significance of Platelet-derived Microparticles in Oral Squamous Cell Carcinoma

作　　者：刘英苗 胡腾龙[2] 李保蓉 于洋[2] LIU Yingmiao;HU Tenglong;LI Baorong(Shunyi Hospital,Beijing Hospital of Traditional Chinese Medicine,Beijing 101300,China)

机构地区：[1]北京中医医院顺义医院口腔科,101300 [2]哈尔滨医科大学附属第一医院口腔颌面外科,150001

出　　处：《医学研究杂志》2023年第7期87-91,共5页Journal of Medical Research

基　　金：北京市自然科学基金资助项目(7224363)。

摘　　要：目的检测口腔鳞状细胞癌(oral squamous cell carcinomas,OSCC)患者外周血中血小板源性微粒(platelet-derived microparticles,PMPs)的含量并探究其在OSCC患者中的临床意义。方法选取OSCC患者及健康人为研究对象。通过扫描电子显微镜观察OSCC患者血浆中的PMPs的表达及用流式细胞仪检测OSCC患者血浆中PMPs的含量。通过凝血功能实验检测OSCC患者PMPs的促凝活性。结果扫描电子显微镜下观察显示,OSCC患者血浆中血小板激活,并产生PMPs,同时OSCC患者血浆中的PMPs水平明显高于健康对照者。PMPs在OSCC患者中的含量与肿瘤最大径、肿瘤TNM分期和颈淋巴结转移有关。OSCC患者外周血中PMPs与凝血相关指标进行Spearman相关性分析,结果表明,纤维蛋白原与PMPs水平呈正相关。结论OSCC患者血浆中PMPs水平和促凝活性随着肿瘤最大径及肿瘤TNM分期的增加而升高,在淋巴结转移的患者中更高,提示PMPs可能作为OSCC进展和转移的一个新的指标。本研究为揭示OSCC凝血紊乱的机制和治疗提供了理论依据和潜在治疗靶点。Objective To measure the level of platelet-derived microparticles(PMPs)in peripheral blood of oral squamous cell carcinoma(OSCC)patients and to investigate their clinical significance in OSCC patients.Methods OSCC patients and healthy subjects were enrolled in the study.The expression of PMPs in OSCC patients′plasma was observed by scanning electron microscope,and the level of PMPs in OSCC patients′plasma was detected by flow cytometry.The procoagulant activity of PMPs was determined by coagulation function test in OSCC patients.Results Electron microscopy could be observed platelet activation and shedding MPs.The plasma levels of PMPs in OSCC patients were significantly higher than in healthy controls.The levels of PMPs in OSCC patients was correlated with the maximum diameter of tumor,TNM stage and cervical lymph node metastasis.The Spearman correlation analysis was performed on PMPs and coagulation related indexes in peripheral blood of OSCC patients,and the results showed that fibrinogen was positively correlated with PMPs levels.Conclusion The plasma PMPs level and procoagulant activity of OSCC patients elevated with the increase of tumor maximum diameter and TNM stage,especially in patients with lymph node metastasis,suggesting that PMPs may be a new indicator of OSCC progression and metastasis.This study provided a new theoretical basis and potential therapeutic targets for revealing the mechanism and treatment of OSCC coagulation disorder.

关 键 词：口腔鳞状细胞癌 血小板源性微粒 凝血功能试验 促凝活性 

分 类 号：R78[医药卫生—口腔医学]