SLFN11基因表达对SW480细胞L-OHP敏感度的影响  

Effect of SLFN11 Expression on the Sensitivity of Oxaliplatin in Colorectal Cancer Cells

作　　者：田力 张宁坤 杨璐 夏菁 彭朝胜 TIAN Li;ZHANG Ningkun;YANG Lu(Day Diagnosis and Treatment Department,The Sixth Medical Center of PLA General Hospital,Beijing 100037,China)

机构地区：[1]中国人民解放军总医院第六医学中心日间诊疗科,北京100048 [2]中国人民解放军总医院第六医学中心心血管内科实验室,北京100048

出　　处：《医学研究杂志》2023年第7期102-106,112,共6页Journal of Medical Research

基　　金：中国人民解放军海军总医院创新培育基金资助项目(CXPY201710)。

摘　　要：目的探讨SLFN11基因表达对奥沙利铂抑制结直肠癌细胞株SW480生长的影响。方法通过SLFN11基因沉默(siRNA)降低SLFN11基因表达水平,并通过RT-qPCR和Western blot法方法鉴定SLFN11基因mRNA和蛋白表达水平;利用MTT实验验证奥沙利铂对SW480细胞株生长增殖的影响;通过流式细胞仪检测SW480细胞周期及细胞凋亡情况。结果siRNA干扰可特异性地显著降低SW480细胞株SLFN11基因的表达和蛋白表达水平,MTT实验发现SLFN11基因沉默组较基因未干扰组,奥沙利铂对SW480细胞株的生长抑制明显减低(P<0.05),而且减弱奥沙利铂对SW480细胞株的细胞凋亡的诱导(P<0.01),减少G 2/M期细胞周期阻滞(P<0.01)。结论SLFN11基因低表达可以降低SW480细胞对奥沙利铂的敏感度,可能预测奥沙利铂治疗结直肠癌细胞的疗效。Objective To discuss the effect of SLFN11 expression on Oxaliplatin inhibiting SW480 cell line growth.Methods The expression of SLFN11 was downregulated by small RNA interference(siRNA)targeting at SLFN11gene.RT-qPCR and Western blot analysis were used to detect the mRNA and protein expression of SLFN11 respectively.Cell proliferation was detected by the MTT assay,and further cell apoptosis rate and cell cycle arrest were determined by flow cytometry.Results The expression level of SLFN11 in SW480 cells was reduced significantly by SLFN11 siRNA.Downregulation of SLFN11 significantly decreased the Oxaliplatin-induced anti-proliferative activity(P<0.05),apoptosis and cell cycle arrest(P<0.01).Conclusion SLFN11 expression could promote Oxaliplatin-induced anti-tumor effect in SW480 cells,suggesting that SLFN11might be used as a predictive biomarker for Oxaliplatin sensitivity in colorectal cancer treatment.

关 键 词：结直肠癌 SLFN11 奥沙利铂 细胞周期 细胞凋亡 

分 类 号：R735[医药卫生—肿瘤]