豆腐果苷对关节失稳模型骨关节炎的作用及机制  

Effect of helicid on osteoarthritis of joint instability model and its mechanism

作　　者：刘格格 刘冉 邱本峰 何雪珺 陈信言 黄蕴哲 贾元威 Shizhang LING 沈杰 LIU Ge-ge;LIU Ran;QIU Ben-feng;HE Xue-jun;CHEN Xin-yan;HUANG Yun-zhe;JIA Yuan-wei;Shizhang LING;SHEN Jie(Graduate School,Wannan Medical College,Wuhu,Anhui 241001,China;The First Affiliated Hospital(Yijishan Hospital)of Wannan Medical College,Wuhu,Anhui 241001,China;The Translational Research Institute for Neurological Disorders,Dept of Neurosurgery of Wannan Medical College,Wuhu,Anhui 241001,China)

机构地区：[1]皖南医学院研究生院,安徽芜湖241001 [2]皖南医学院第一附属医院(弋矶山医院),安徽芜湖241001 [3]皖南医学院神经系统疾病转化医学研究中心,神经外科学系,安徽芜湖241001

出　　处：《中国药理学通报》2023年第8期1457-1463,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81503236);弋矶山医院引进人才科研基金资助项目(No YR201913,YR202015);皖南医学院弋矶山医院科研能力“高峰”培育计划(No GF2019J05,GF2019J06);皖南医学院弋矶山医院科技创新团队“攀峰”培育计划(No KPF2019016)。

摘　　要：目的研究豆腐果苷对关节失稳模型骨关节炎(osteoarthritis,OA)的治疗作用,探讨豆腐果苷治疗OA的作用机制。方法通过切除大鼠膝关节内侧半月板的方法(medial meniscectomy,MMx)建立OA大鼠模型。术后给予豆腐果苷治疗后,通过HE和番红O-固绿染色法观察膝关节软骨组织的组织病理学变化;免疫组化法检测膝关节软骨和滑膜组织中瞬时受体电位香草酸亚型1(Trpv1)表达;Western blot法检测大鼠膝关节滑膜组织中Trpv1蛋白的表达情况。结果HE和番红O-固绿染色法显示,豆腐果苷治疗明显减缓膝关节软骨的退变情况。免疫组化结果显示,豆腐果苷明显降低膝关节软骨和滑膜中Trpv1蛋白的表达水平;Western blot结果证实豆腐果苷下调膝关节滑膜组织中Trpv1蛋白的表达量。结论豆腐果苷能明显抑制MMx诱导的关节软骨的破坏和软骨基质的丢失,从而发挥治疗OA作用。Aim To study the therapeutic effect of helicid on osteoarthritis(OA)of joint instability model,and explore the mechanism of helicid in the treatment of OA.Methods A rat knee model of OA was established by the medial meniscectomy(MMx)method.After treatment with helicid,HE and safranin O/fast green staining methods were used to observe the histopathological changes of rat knee articular cartilage;Western blot was used to detect the protein expression level of Trpv1 in rat synovial tissue.Immunohistochemical staining was used to examine the expression of Trpv1 in rat knee articular cartilage and synovial tissues.Results Helicid significantly slowed down the degeneration of rat knee articular cartilage as shown by HE and safranin O/fast green staining.Western blot results showed that helicid down-regulated the expression of Trpv1 in rat synovial tissue examined.Immunohistochemical results showed that helicid significantly reduced the expression of Trpv1 in both of knee articular cartilage and synovial tissues.Conclusions Helicid prominently decreases MMx-induced articular cartilage damage and cartilage matrix loss,thereby exerting a therapeutic effect on OA.

关 键 词：豆腐果苷 MMX 骨关节炎 关节失稳模型 TRPV1 滑膜 

分 类 号：R-332[医药卫生] R285.5R322.72R684.3R916.4