富钙微环境对牙周膜干细胞增殖及迁移能力的影响  

Effects of Ca^(2+) on proliferation and migration of human periodontal ligament stem cells

作　　者：齐芳芳[1,4] 李雅 郝君玲[1,4] 张立亚 谷希 马文盛 李颖辉 QI Fangfang;LI Ya;HAO Junling(Department of Endodontics,Hospital of Stomatology Hebei Medical University,Hebei,Shijiazhuang 050000,China;不详)

机构地区：[1]河北医科大学口腔医院牙体牙髓科,石家庄市050000 [2]河北医科大学口腔医院牙周科,石家庄市050000 [3]河北医科大学口腔医院口腔正畸科,石家庄市050000 [4]河北省口腔医学重点实验室

出　　处：《河北医药》2023年第14期2117-2120,共4页Hebei Medical Journal

基　　金：河北省医学科学研究课题计划(编号:20221446,20191077);河北省教育厅高等学校科学研究计划(编号:ZD2017054)。

摘　　要：目的探讨含有不同浓度钙离子(Ca^(2+))的培养基对人牙周膜干细胞(human periodontal ligament stem cells,hPDLSCs)增殖与迁移能力的影响。方法用含0 mmol/L、5 mmol/L、10 mmol/L、15 mmol/L钙离子的完全培养基干预第3代hPDLSCs,其中0mM组作为对照组,通过CFU法、MTT法及划痕实验分别于相应时间点检测其克隆形成能力、细胞增殖能力及细胞迁移能力,并将各组间结果进行对比。结果在含钙离子培养基中,与对照组对比,hPDLSCs克隆形成能力、细胞增殖能力、细胞迁移能力均显示不同程度的增加。CFU结果显示5 mmol/L、10 mmol/L、15 mmol/L钙离子组的克隆形成能力明显优于对照组(P<0.05);MTT检测结果显示自第3天开始含钙离子组hPDLSCs的增殖能力优于对照组(P<0.05),第5天、7天时,5 mmol/L、10 mmol/L组的增殖能力优于对照组及15 mmol/L组(P<0.05);划痕实验结果显示5 mmol/L、10 mmol/L钙离子组的迁移能力优于对照组(P<0.05),在钙离子浓度增至15 mmol/L时,其迁移能力显著下降,低于0 mmol/L、5 mmol/L、10 mmol/L组(P<0.05)。结论在体外实验中,含一定浓度钙离子培养基可促进hPDLSCs的增殖与迁移能力。Objective To investigate the effect of Ca^(2+)on the proliferation and migration of human periodontal ligament stem cells(hPDLSCs).Methods The third-generation hPDLSCs were induced with 0 mmol/L(control),5mmol/L,10mmol/L,and 15mmol/L Ca^(2+).CFU,MTT assay and scratch test were performed to detect the clone formation,cell proliferation and cell migration at each time points.Results Compared with the control group,the clonogenesis,proliferation and migration ability increased in Ca^(2+)-induced hPDLSCs.CFU results showed that the cloning ability of hPDLSCs treated with 5mmol/L,10mmol/L and 15mmol/L Ca^(2+)was significantly higher than that in the control group(P<0.05).MTT assay showed that the proliferation ability of Ca^(2+)-induced hPDLSCs was better than that in control group from the 3rd day(P<0.05),and the proliferation ability of hPDLSCs induced with 5mmol/L and 10mmol/L Ca^(2+)was better than that in control group and 15mmol/L group on the 5th and 7th day(P<0.05).The results of scratch test showed that the migration ability of hPDLSCs induced with 5mmol/L and 10mmol/L Ca^(2+)were significantly better than that in the control group(P<0.05),which significantly decreased by the treatment of 15mmol/L Ca^(2+)compared with the remaining groups.Conclusion Calcium can promote the proliferation and migration of hPDLSCs in vitro.

关 键 词：牙周膜干细胞 钙离子 增殖 迁移 

分 类 号：R781.4[医药卫生—口腔医学]