丙泊酚对脂多糖诱导肝癌细胞炎症反应、增殖、黏附、侵袭的影响及机制  

Effect and mechanism of propofol on lipopolysaccharide-induced inflammation response,proliferation,adhesion and invasion of hepatocellular carcinoma cells

作　　者：齐少霞 杨栋宝 周翼 武倩[1] 王晓东 QI Shaoxia;YANG Dongbao;ZHOU Yi;WU Qian;WANG Xiaodong(The Second Department of Anesthesiology,Cangzhou Hospital of Integrated Chinese and Western Medicine,Cangzhou 061000,Hebei Province,China)

机构地区：[1]沧州中西医结合医院麻醉二科,河北沧州061000

出　　处：《新乡医学院学报》2023年第8期712-720,共9页Journal of Xinxiang Medical University

基　　金：河北省医学科学研究课题计划项目(编号:20220692)。

摘　　要：目的探讨丙泊酚对脂多糖(LSP)诱导的人肝癌细胞炎症反应、增殖、凋亡、黏附、侵袭的影响及机制。方法将对数生长期肝癌MHCC97H细胞按随机数字表法分为对照组、LPS组和低剂量丙泊酚、中剂量丙泊酚、高剂量丙泊酚组。对照组细胞不做干预;LPS组细胞给予1 mg·L^(-1)LPS干预;低剂量丙泊酚组、中剂量丙泊酚组和高剂量丙泊酚组均给予1 mg·L^(-1)LPS干预24 h后,再分别给予12.5、25.0和50.0μmol·L^(-1)丙泊酚干预。采用酶联免疫吸附试验检测各组细胞培养液中炎症因子白细胞介素1β(IL-1β)和肿瘤坏死因子-α(TNF-α)表达水平,采用细胞计数试剂盒-8测定各组细胞活力,采用5-乙炔基-2′脱氧尿嘧啶核苷法检测各组细胞增殖情况,采用Hoechst 33258染色法检测各组细胞凋亡情况,采用细胞黏附实验测定各组黏附细胞数,采用Transwell小室检测各组细胞的侵袭能力,采用蛋白免疫印迹法测定细胞周期素D1(Cyclin D1)、caspase-3、E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)和纤连蛋白(FN)表达水平。结果LPS组IL-1β水平、TNF-α水平、细胞活力、细胞增殖率、黏附细胞数、侵袭细胞数及Cyclin D1、N-cadherin、Vimentin和FN水平显著高于对照组(P<0.05),细胞凋亡率和caspase-3、E-cadherin水平显著低于对照组(P<0.05)。中剂量丙泊酚组、高剂量丙泊酚组TNF-α水平、细胞活力、细胞增殖率、黏附细胞数、侵袭细胞数及Cyclin D1、N-cadherin、Vimentin水平显著低于对照组(P<0.05),细胞凋亡率、caspase-3、E-cadherin蛋白表达水平显著高于对照组(P<0.05);中剂量丙泊酚组、高剂量丙泊酚组与对照组IL-1β水平比较差异无统计学意义(P>0.05);高剂量丙泊酚组FN水平显著低于对照组(P<0.05),中剂量丙泊酚组与对照组FN蛋白表达水平比较差异无统计学意义(P>0.05)。低剂量丙泊酚组细胞侵袭数、E-cadherin水平显著低于对照组,CyclObjective To investigate the effect and mechanism of propofol on lipopolysaccharide(LSP)-induced inflammatory response,proliferation,apoptosis,adhesion and invasion of human hepatocellular carcinoma cells.Methods Hepatocellular carcinoma MHCC97H cells in logarithmic growth phase were divided into control group,LPS group and low-dose propofol group,medium-dose propofol group and high-dose propofol group according to the random number table method.The cells in the control group were not intervened;the cells in the LPS group were intervened with 1 mg·L^(-1)LPS;and the cells in the low-dose propofol group,medium-dose propofol group and high-dose propofol group were treated with 1 mg·L^(-1)LPS for 24 hours,then treated with 12.5,25.0 and 50.0μmol·L^(-1)propofol,respectively.The expression levels of inflammatory factors interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in cell culture medium in each group were detected by enzyme-linked immunosorbent assay;the cell viability in each group was measured by cell counting kit-8;the cell proliferation in each group was detected by 5-ethynyl-2′-deoxyuridine method;the cell apoptosis in each group was detected by Hoechst 33258 staining;the number of adherent cells in each group was determined by cell adhesion assay;the invasion ability in each group was determined by Transwell chamber;the expression levels of Cyclin D1,caspase-3,E-cadherin,N-cadherin,Vimentin and fibronectin(FN)in each group were detected by Western blot.Results The IL-1βlevel,TNF-αlevel,cell viability,cell proliferation rate,number of adherent cells,number of invasive cells,and the levels of Cyclin D1,N-cadherin,Vimentin and FN protein in the LPS group were significantly higher than those in the control group(P<0.05);and the cell apoptosis rate,caspase-3 and E-cadherin level were significantly lower than those in the control group(P<0.05).The TNF-αlevel,cell viability,proliferation rate,number of adherent cells,number of invasive cells and the levels of Cyclin D1,N-cadherin,and Vimentin in t

关 键 词：肝癌 丙泊酚 脂多糖 增殖 凋亡 黏附 侵袭 上皮间质转化 

分 类 号：R735.7[医药卫生—肿瘤]