miR-3619-5p、LINC00857在原发性肝细胞癌血清中表达水平及其临床诊断价值分析  

Expression levels and clinical diagnostic value of miR-3619-5p and LINC00857 in serum of hepatocellular carcinoma

作　　者：慕喜喜 李静[2] 张玄[3] 高小鹏[1] 刘保荣[1] 钟岳[4] MU Xixi;LI Jing;ZHANG Xuan(Department of General Surgery 2,Xi'an Central Hospital,Shaanxi,Xi'an 710003,China;不详)

机构地区：[1]西安市中心医院普外二科,陕西西安710003 [2]西安市中心医院科教科,陕西西安710003 [3]西京医院肝胆外科 [4]陕西省第二人民医院肝胆普外科

出　　处：《临床外科杂志》2023年第6期572-575,共4页Journal of Clinical Surgery

摘　　要：目的探究微小RNA(miR)-3619-5p、长链非编码RNA(LncRNA)LINC00857在原发性肝细胞癌(HCC)血清中的表达水平及其临床诊断价值。方法选取HCC病人78例、肝脏良性病变病人76例、慢性肝病病人78例分别为HCC组、良性病变组、慢性肝病组,另选取体检健康者78例为对照组。比较各组血清miR-3619-5p、LncRNA LINC00857、甲胎蛋白(AFP)水平;分析血清miR-3619-5p、LncRNA LINC00857、AFP对HCC的诊断价值。结果对照组、慢性肝病组、良性病变组、HCC组病人血清miR-3619-5p[(1.05±0.35)、(0.74±0.25)、(0.72±0.24)、(0.46±0.15)、]及LncRNA LINC00857[(1.01±0.34)、(1.62±0.54)、(1.66±0.55)、(2.95±0.98)]、AFP[(3.13±1.14)μg/L、(48.86±16.29)μg/L、(50.23±18.74)μg/L、(301.25±117.23)μg/L]水平比较,差异有统计学意义(P<0.05);血清miR-3619-5p、LncRNA LINC00857、AFP诊断HCC的曲线下面积(AUC)分别为0.870、0.868、0.701,血清miR-3619-5p、LncRNA LINC00857联合诊断HCC的AUC为0.956,灵敏度为94.9%,特异度为86.4%。结论HCC病人血清miR-3619-5p表达较低,LncRNA LINC00857表达较高,miR-3619-5p、LncRNA LINC00857联合可能更有助于临床诊断HCC。Objective To investigate the expression levels of microRNA-3619-5p(miR-3619-5p)and long non-coding RNA(LncRNA)LINC00857 in serum of hepatocellular carcinoma(HCC)and their clinical diagnostic value.Methods 78 patients with HCC,76 patients with benign liver lesions,and 78 patients with chronic liver disease were taken as HCC group,benign lesion group,and chronic liver disease group,respectively,another 78 healthy subjects who underwent physical examination were taken as the control group.The serum levels of miR-3619-5p,LncRNA LINC00857 and alpha-fetoprotein(AFP)in each group were compared;the diagnostic value of serum miR-3619-5p,LncRNA LINC00857 and AFP for HCC was analyzed.Results Comparison of serum miR-3619-5p[(1.05±0.35),(0.74±0.25),(0.72±0.24),(0.46±0.15)]and LncRNA LINC00,857[(1.01±0.34),(1.62±0.54),(1.66±0.55),(2.95±0.98)],AFP[(3.13±1.14)μg/L,(48.86±16.29)μg/L,(50.23±18.74)μg/L,(301.25±117.23)μg/L]levels in patients with control group,chronic liver disease group,benign disease group,and HCC group,the difference was statistically significant(P<0.05).The areas under the curve(AUC)of serum miR-3619-5p,LncRNA LINC00857,and AFP for the diagnosis of HCC were 0.870,0.868,and 0.701,respectively,the AUC of serum miR-3619-5p combined with LncRNA LINC00857 to diagnose HCC was 0.956,the sensitivity was 94.9%,and the specificity was 86.4%.Conclusion The expression level of serum miR-3619-5p in HCC patients is low,while the expression level of LncRNA LINC00857 is high.The combination of miR-3619-5p and LncRNA LINC00857 may be more helpful for clinical diagnosis of HCC.

关 键 词：微小RNA-3619-5p 原发性肝细胞癌 长链非编码RNA LINC00857 诊断价值 

分 类 号：R735.7[医药卫生—肿瘤]