短时程光照对小鼠SCN和LHB谷氨酸受体表达的影响  

Effects of an ultradian light on periodic expression of glutamate receptors in the SCN and LHB

作　　者：李佳逸 金巧玲 王烈成 程娟 Li Jiayi;Jin Qiaoling;Wang Liecheng;Cheng Juan(Dept of Physiology,School of Basic Medical Sciences,Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学基础医学院生理学教研室,合肥230032

出　　处：《安徽医科大学学报》2023年第8期1306-1312,共7页Acta Universitatis Medicinalis Anhui

基　　金：安徽省高等学校科学研究项目(编号:2022AH050783)。

摘　　要：目的探究短时程光照对视交叉上核(SCN)和外侧缰核(LHB)谷氨酸受体表达的影响。方法利用T7(3.5 h/3.5 h光暗循环)建立短时程光周期模型组和T24(12 h/12 h光暗循环)正常光建立对照组,通过蛋白免疫印迹(Western blot)的方法对SCN和LHB脑区关键受体蛋白的表达进行分析,包括谷氨酸能受体、垂体腺苷酸环化酶激活肽(PACAP)受体以及下游信号分子。结果Western blot结果显示,与T24相比,T7下SCN在授时因子时间(ZT)1、ZT 5的α-氨基-3-羟基-5-甲基-4-异恶唑丙酸受体亚型2(GluR2)的表达增多(P<0.05,P<0.01),T7组GluR2总体表达比T24组增多(P<0.01),而LHB中T7组GluR2总体表达比T24组减少(P<0.01),与T24光周期相比,T7光周期下SCN的N-甲基-D-天冬氨酸受体亚型2(NR2B)的总体表达上升(P<0.05),T7光周期下LHB的磷酸化的细胞外调蛋白激酶(P-ERK)的总体表达上升(P<0.05)。结论T7光周期会导致SCN中GluR2和NR2B表达增多、LHB中GluR2受体表达降低及P-ERK的表达升高。Objective To explore the effects of an ultradian light cycle on the periodic expression of glutamate receptors in the suprachiasmatic nucleus and the lateral habenula nucleus.Methods An ultradian light cycle T7(3.5 hours/3.5 hours light:dark)was used to establish an ultradian light cycle model group and T24(12 hours/12 hours light:dark)was used to establish control group.The expression of key proteins in the brain regions of the suprachiasmatic nucleus(SCN)and the lateral habenula(LHB)of the hypothalamus were analyzed by Western blot.Including glutaminergic receptors,pituitary adenylate cyclase activating polypeptide(PACAP)receptors,and downstream signaling molecules.Results Western blot results showed that the expression of α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor subunit 2(GluR2)in SCN under Zeitgeber time(ZT)1 and ZT 5 increased under an ultradian light cycle compared with normal photoperiod(P<0.05,P<0.01),the overall expression of CluR2 in T7 group was higher than that in T24 group(P<0.01),the overall expression of GluR2 in LHB group T7 was lower than that in T24 group(P<0.01).Compared with T24 photoperiod,the overall expression of N-methyl-D-aspartic acid receptor subunit 2(NR2B)in SCN increased under T7 photoperiod(P<0.05),the overall expression of phosphorylation of extracellular signal-related kinase(P-ERK)in LHB significantly increased under T7 photoperiod(P<0.05).Conclusion An ultradian light cycle would cause an up-regulating of GluR2 and NR2B expression,a down-regulating of GluR2 expression and up-regulating P-ERK expression in LHB.

关 键 词：短时程光照 外侧缰核 视交叉上核 谷氨酸受体 

分 类 号：R338.6[医药卫生—人体生理学]