GRB2 rs8082005、RXRA rs3849222位点与延边地区EOPE易感性的关系  

Relationship between GRB2 rs8082005,RXRA rs3849222 and susceptibility to early-onset preeclampsia in Yanbian area

作　　者：蔡宛轩 刘霞 金光[1] 董春玉 杨恩月 Cai Wanxuan;Liu Xia;Jin Guang;Dong Chunyu;Yang Enyue(Laboratory of Pathology and Pathophysiology of Yanbian University,Yanji 133002;Gynaecology and Obstetrics,Affiliated Hospital of Yanbian University,Yanji 133002;Clinical Pharmacology Institute,Affiliated Hospital of Yanbian University,Yanji 133002)

机构地区：[1]延边大学病理学与病理生理学实验室,延吉133002 [2]延边大学附属医院妇产科,延吉133002 [3]延边大学附属医院临床药理机构,延吉133002

出　　处：《安徽医科大学学报》2023年第8期1403-1407,共5页Acta Universitatis Medicinalis Anhui

基　　金：吉林省自然科学基金(编号:20190201056JC、YDZJ202201-ZYTS175)。

摘　　要：目的分析基因的多个位点与妊娠高血压的早发型子痫前期(EOPE)风险的联系。方法自延边地区居住10年以上、经医院诊断为EOPE且无其他疾病的患者382例患者中随机选取192例为病例组。同期在该医院自然分娩的健康人群中随机选取192例作为对照组。依据PCR-RFLP法来测定其中具体的基因型及等位基因分布等信息,依据非条件Logistic方法来获得比值比(OR)及95%可信区间(CI),确认各类基因型的风险度。结果GRB2 rs8082005位点存在T和C两种等位基因,TC、TT和CC基因型;RXRA rs3849222位点存在T和C两种等位基因,TC、TT和CC基因型。经非条件Logistic回归分析,在GRB2 rs8082005位点中,与TT基因型相比,CC基因型更易患EOPE(OR=3.155,95%CI=1.513~6.579,P=0.002);在显性模型中,相比于携带TT+TC基因型患者,携带CC基因型患者增加EOPE发病风险(OR=3.000,95%CI=1.495~6.022,P=0.002)。在RXRA rs3849222位点中,与CC基因型相比,TT基因型更易得EOPE(OR=2.031,95%CI=1.077~3.820,P=0.028);在隐形模型中,相比于携带CC+CT基因型患者,携带TT基因型患者EOPE发病风险增加(OR=2.549,95%CI=1.421~4.573,P=0.002)。结论GRB2 rs8082005、RXRA rs3849222位点单核苷酸多态性(SNP)与延边地区孕妇患妊娠高血压的EOPE风险有明显相关性。Objective To analyze the association between multiple loci of genes and the risk of early-onset preeclampsia(EOPE)with pregnancy induced hypertension.Methods Among 382 EOPE patients who had lived in Yanbian area for more than 10 years,192 patients were randomly selected as case group.At the same time,192 cases of natural delivery in the hospital were randomly selected as the control group.PCR-RFLP method was used to determine the specific genotype and allele distribution information,and non-conditional Logistic method was used to obtain odds ratio(OR)and 95%confidence interval(CI)to confirm the risk of various genotypes.Results There were two alleles of T and C at the GRB2 rs8082005 locus,TC,TT,and CC genotypes.There were two alleles of T and C at the RXRA rs3849222 locus,TC,TT,and CC genotypes.Through unconditional logistic regression analysis,in the GRB2 rs8082005 locus,compared with the TT genotype,the CC genotype was more susceptible to EOPE(OR=3.155,95%CI=1.513-6.579,P=0.002).In the explicit model,compared to patients with TT+TC genotype,patients with CC genotype increased the risk of EOPE(OR=3.000,95%CI=1.495-6.022,P=0.002).In the RXRA rs3849222 locus,compared with the CC genotype,the TT genotype was more susceptible to EOPE(OR=2.031,95%CI=1.077-3.820,P=0.028).In the invisible model,compared to patients with CC+CT genotype,patients with TT genotype had an increased risk of EOPE(OR=2.549,95%CI=1.421-4.573,P=0.002).Conclusion There is a significant correlation between single nucleotide polymorphism(SNP)at the GRB2 rs8082005 and RXRA rs3849222 loci and the risk of EOPE in pregnant women with gestational hypertension in Yanbian area.

关 键 词：早发型子痫前期 单核苷酸多态性 易感性 

分 类 号：R365[医药卫生—病理学]