TIMP-1在大鼠不可复性牙髓炎中的抗炎作用  

Anti-inflammatory effect of TIMP-1 in irreversible pulpitis in rats

作　　者：唐颖 李午丽[1] 杨琪 王璐 李颂[1] Tang Ying;Li Wuli;Yang Qi;Wang Lu;Li Song(Stomatological College of Anhui Medical University,The Affiliated Stomatological Hospital of Anhui Medical University,Key Lab.of Oral Diseases Research of Anhui Province,Hefei 230032)

机构地区：[1]安徽医科大学口腔医学院,安徽医科大学附属口腔医院,安徽省口腔疾病研究重点实验室,合肥230032

出　　处：《安徽医科大学学报》2023年第8期1408-1412,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省高校自然科学研究项目(编号:KJ2020A0164)。

摘　　要：目的研究金属蛋白酶抑制剂1(TIMP-1)在大鼠不可复性牙髓炎模型活髓保存治疗中的抗炎作用。方法对8~10周龄雄性大鼠上颌第一磨牙采用髓腔开放法建立不可复性牙髓炎模型,髓腔开放24、36、48 h后进行牙髓切断术,用TIMP-1覆盖牙髓断面(TIMP-1组),生理盐水作为对照(NS组),玻璃离子填充窝洞。给药后24、48 h分别进行组织学及免疫组化分析。结果HE染色切片的组织病理学定量分析结果显示,TIMP-1组的根髓炎性细胞反应、根髓组织形态改变、根髓坏死范围的炎症评分高于NS组(P<0.001或P<0.01)。免疫荧光染色(CD68标记)切片显示,TIMP-1组CD68免疫荧光强度高于NS组(P<0.001)。结论TIMP-1在大鼠不可复性牙髓炎模型中无抗炎作用,且加重了根髓的炎症程度。Objective To evaluate the anti-inflammatory effect of tissue inhibitor of metalloprotease(TIMP1)on a rat model of irreversible pulpitis.Methods An irreversible pulpitis model was established by pulp exposure on maxillary first molars of 8-10 weeks old male rats,and pulpotomy was performed at 24 h,36 h and 48 h after pulp exposure,with TIMP-1 covering the pulp section(TIMP-1 group)and saline as control(NS group).Coronal obturation were performed with glass-ionomer cement.Histological and immunohistochemical analysis were performed at 24 and 48 h after administration.Results Quantitative histopathological analysis of HE-stained sections showed that the inflammation score(inflammatory cellular response of the root pulp,morphological changes in root pulp tissue and extent of root pulp necrosis)of the pulp were higher in the TIMP-1 group than those in the NS group(P<0.001 or P<0.01).Immunofluorescence staining(CD68-labeled)sections showed that the intensity of CD68 immunofluorescence was higher in the TIMP-1 group than that in the NS group(P<0.001).Conclusion TIMP-1 had no anti-inflammatory effect in a rat model of irreducible pulpitis and increased the degree of inflammation in the root pulp.

关 键 词：不可复性牙髓炎 活髓保存治疗 金属蛋白酶组织抑制剂1 髓腔开放法 

分 类 号：R781.31[医药卫生—口腔医学]