初级纤毛在口腔黏膜创伤愈合中的作用研究  

Study on the role of primary cilia in the healing of oral mucosal wound

作　　者：王欣宇 孙瑶[1] WANG Xinyu;SUN Yao(Department of Implantology,School&Hospital of Stomatology,Tongji University,Shanghai Engineering Re-search Center of Tooth Restoration and Regeneration,Shanghai 200072,China)

机构地区：[1]同济大学口腔医学院·同济大学附属口腔医院种植科,上海牙组织修复与再生工程技术研究中心,上海200072

出　　处：《口腔医学》2023年第7期584-591,共8页Stomatology

基　　金：国家自然科学基金(82270963)。

摘　　要：目的探究初级纤毛的增龄性变化与口腔黏膜创伤愈合的相关性。方法对不同年龄阶段的小鼠颊侧口腔黏膜构建创伤模型,分别在创伤后1、3、5 d收样。通过HE染色观察愈合过程,MASSON染色观察组织重塑情况,免疫荧光染色观察再上皮化过程,并通过EdU标记观察创伤愈合过程中细胞增殖水平的差异。通过构建口腔黏膜上皮初级纤毛关键分子Ift140条件性敲除小鼠,观察敲除组与对照组小鼠口腔黏膜创伤愈合速度的差异。结果老年小鼠颊侧口腔黏膜上皮自我更新能力下降,初级纤毛出现率降低;创伤后黏膜愈合延迟,角质形成细胞增殖速率降低,向伤口床迁移速度减慢。在口腔黏膜上皮K14阳性细胞中条件性敲除Ift140干预初级纤毛形成后,创伤愈合过程延迟。结论小鼠口腔黏膜上皮初级纤毛的增龄性变化延缓口腔黏膜创伤愈合。Objective To investigate the correlation between ageing changes in primary cilia and oral mucosal wound healing.Meth⁃ods A wound model was constructed for buccal oral mucosa of mice at different ages,and samples were collected at 1,3 and 5 days after modeling.The healing process was observed by HE staining,the tissue remodeling by MASSON staining,the re⁃epithelialization process by immunofluorescence staining,and the difference of cell proliferation level during the wound healing process by EdU labeling.By constructing mice with specific knockout of the primary cilia key molecule Ift140 in oral mucosal epithelial,the difference of oral mucosal wound healing speed between knockout and control mice was observed.Results The oral mucosal epithelium of aged mice had decreased self⁃renewal ability and the occurrence rate of primary cilia was reduced.Mucosal healing was delayed after wound,and the proliferation rate of keratinocytes was reduced and the migration rate to the wound bed was slowed.Conditional knockdown of Ift140 in oral mucosal epithelial K14⁃positive cells interfered with primary cilia formation and delayed the wound healing process.Conclusion Age⁃related changes in primary cilia of mouse oral mucosal epithelium delay oral mucosal wound healing.

关 键 词：口腔黏膜 创伤愈合 再上皮化 初级纤毛 衰老 

分 类 号：R781.59[医药卫生—口腔医学]