Comparison of Microdroplet Digital PCR Assays with Real-time Fluorescence Quantitative PCR for Clostridioides difficile Detection  

作　　者：YIN Cai Hong SONG Zhan Yun LIU Xing Xing WANG Xiao Mu WANG Ying GAO Cheng Cheng SONG Xiu Ling FENG Xin 

机构地区：[1]College of Public Health,Jilin University,Changchun 130021,Jilin,China [2]Changchun customs Technology Center,Changchun130062,Jilin,China [3]CollegeofVeterinaryMedicine,JilinUniversity,Changchun130062,Jilin,China [4]College of Basic Medical Sciences,Jilin University,Changchun 130021,Jilin,China

出　　处：《Biomedical and Environmental Sciences》2023年第7期653-657,共5页生物医学与环境科学（英文版）

基　　金：funded by the Scientific and Technological Research Project of Jilin Province[grant numbers:20220203146SF,YDZJ202302CXJD054,20210101365JC];the Health Commission of Jilin Province[grant number:2019Q011];the Development of Education in Jilin Province,China[grant number:JJKH20211220KJ].

摘　　要：Clostridioides difficile(C.difficile),is an anaerobic Gram-positive bacterium found in the human intestine.Prolonged use of antibacterial drugs can result in C.difficile infection(CDI)[1],which is one of the most important hospital-acquired diseases affecting all wards.Isolation-and-culture-based identification and the cell cytotoxicity neutralization assay(CCNA)are considered the“gold standard”for detecting toxins or pathogenic strains[2],with long and time-consuming incubation times.Fluorescent quantitative polymerase chain reaction(PCR)is commonly used in clinical laboratory tests[3].However,the sensitivity,accuracy,and resolution of detection are limited by low-copy target gene molecules and subtle differences in template concentration.As C.difficile is distributed in feces in the natural environment and the sample matrix is complex,it is difficult to detect pathogens at low levels.Accurately quantifying C.difficile and implementing effective measures to combat C.difficile infections are of utmost importance.

关 键 词：INTESTINE DRUGS TEMPLATE 

分 类 号：R446.5[医药卫生—诊断学]