类球红细菌基因组密码子偏好性分析  

Analysis of Codon Usage Bias in the Genome of Rhodobacter sphaeroides

作　　者：张艳艳 江贤章[1,2] ZHANG Yanyan;JIANG Xianzhang(College of Life Science,Fujian Normal University,Fuzhou 350117,China;National Joint Engineering Research Center of Industrial Microbiology and Fermentation Technology,Fuzhou 350117,China)

机构地区：[1]福建师范大学生命科学学院,福建福州350117 [2]工业微生物发酵技术国家地方联合工程研究中心,福建福州350117

出　　处：《生物化工》2023年第3期83-86,共4页Biological Chemical Engineering

基　　金：福建省自然科学基金项目(2020J01177)。

摘　　要：密码子偏好性可影响外源基因在其体内的表达,为了解类球红细菌的密码子使用模式,本研究利用CodonW对类球红细菌2.4.1的基因组进行分析。结果显示,类球红细菌密码子的使用偏好性主要受选择压力的影响,在类球红细菌使用频率较高的27个密码子中,其中GC结尾的密码子占85.19%。同时确定了15个高表达优越密码子,除终止密码子外,其余都以GC结尾,表明类球红细菌中的密码子使用偏向于GC结尾的同义密码子。类球红细菌与大肠杆菌和谷氨酸棒状杆菌的密码子在使用偏好性上差别较小,与酿酒酵母的差异相对较大。本研究对开发类球红细菌基因表达宿主以及提高外源基因在类球红细菌中的表达提供理论基础。The codon preference may affect the expression of foreign genes.To understand the codon usage pattern of R.sphaeroides,CodonW is used to analyze the genome of R.sphaeroides 2.4.1.The results show that the codon usage bias of R.sphaeroides is mainly affected by the selective pressure.Among the 27 codons frequently used by R.sphaeroides,the codons ending at GC accounted for 85.19%.In addition,fifteen high-expression superior codons are determined,among which,except for the stop codon,the rest of the codons all ended with GC.The analysis also show that the codon usage bias in R.sphaeroides is similar to that in Escherichia coli and Corynebacterium glutamicum while it is relatively different from that in Saccharomyces cerevisiae.This study provides a theoretical basis for developing efficient hosts for gene expression in R.sphaeroides species as well as improving the expression of foreign genes in R.sphaeroides.

关 键 词：类球红细菌 密码子偏好 最优密码子 

分 类 号：Q939.9[生物学—微生物学]