去铁胺减少蛛网膜下腔出血大鼠神经细胞凋亡  

Deferriamine reduces nerve cell apoptosis in rats with subarachnoid hemorrhage

作　　者：张冲 付丽丽 费爽 郝广志 陈立刚 ZHANG Chong;FU Lili;FEI Shuang;HAO Guangzhi;CHEN Ligang(Department of Neurosurgery,General Hospital of Northern Theater Command of Chinese People's Liberation Army,Shenyang 110016,China)

机构地区：[1]中国人民解放军北部战区总医院神经外科,沈阳110016

出　　处：《神经解剖学杂志》2023年第3期321-325,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(82071481)。

摘　　要：目的:研究去铁胺(DFX)对蛛网膜下腔出血(SAH)大鼠的神经保护作用。方法:利用颈动脉穿刺法建立SAH大鼠模型。神经功能评分评价DFX对SAH大鼠神经功能的影响,利用商品化试剂盒检测脑脊液中游离铁的含量。末端DNA转移酶dUTP缺口末端标记法(TUNEL)染色检测大鼠皮层细胞凋亡。结果:与SAH组大鼠相比,DFX治疗后可以显著改善大鼠的神经功能,同时降低脑脊液中游离铁的水平,并减少细胞凋亡。结论:DFX能够减少SAH大鼠铁超载导致的细胞凋亡并改善神经功能。Objective:To study the neuroprotective effect of deferriamine(DFX) on subarachnoid hemorrhage(SAH) in rats.Methods:The rat model of SAH was established by carotid artery puncture.Neurological function score was used to evaluate the effect of DFX on the neurological function of SAH rats,and the content of free iron in cerebrospinal fluid was detected by commercial kit.Cell apoptosis was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling(TUNEL) staining.Results:Compared with SAH group,DFX treatment can significantly improve the neurological function of rats,reduce the level of free iron in cerebrospinal fluid,and reduce cell apoptosis.Conclusion:DFX can reduce apoptosis induced by iron overload and improve neurological function in SAH rats.

关 键 词：蛛网膜下腔出血 去铁胺 铁 凋亡 大鼠 

分 类 号：R743.35[医药卫生—神经病学与精神病学]