Morphofunctional characterization of hemocytes in black soldier fly larvae  

作　　者：Daniele Bruno Aurora Montali Marzia Gariboldi Anna Katarzyna Wronska Agata Kaczmarek Amr Mohamed Ling Tian Morena Casarteli Gianluca Tettamanti 

机构地区：[1]Department of Biotechnology and Life Sciences,University of Insubria,Varese,Italy [2]Host Parasites Molecular Interaction Research Unit,Witold Stefanski Institute of Parasitology,Polish Academy of Sciences,Warsaw,Poland [3]Department of Entomology,Faculty of Science,Cairo University,Giza,Egypt [4]Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding,Guangdong Provincial Sericulture and Mulberry Engineering Research Center,College of Animal Science,South China Agricultural University,Guangzhou,China [5]Department of Biosciences,University of Milano,Milano,Italy [6]Interuniversity Centerfor Studies on Bioinspired Agro-environmental Technology(BAT Center),University of Napoli Federico ll,Portici,Italy

出　　处：《Insect Science》2023年第4期912-932,共21页昆虫科学（英文版）

基　　金：supported by Fondazione Cariplo(grant number 2020-0900).

摘　　要：In insects,the cell-mediated immune response involves an active role of hemo-cytes in phagocytosis,nodulation,and encapsulation.Although these processes have been well documented in multiple species belonging to different insect orders,information con-cerning the immune response,particularly the hemocyte types and their specific function in the black soldier fly Hermetia illucens,is still limited.This is a serious gap in knowledge given the high economic relevance of H.illucens larvae in waste management strategies and considering that the saprophagous feeding habits of this dipteran species have likely shaped its immune system to efficiently respond to infections.The present study repre-sents the first detailed characterization of black soldier fly hemocytes and provides new insights into the cell-mediated immune response of this insect.In particular,in addition to prohemocytes,we identified five hemocyte types that mount the immune response in the larva,and analyzed their behavior,role,and morphofunctional changes in response to bac-terial infection and injection of chromatographic beads.Our results demonstrate that the circulating phagocytes in black soldier fly larvae are plasmatocytes.These cells also take part in nodulation and encapsulation with granulocytes and lamellocyte-like cells,devel-oping a starting core for nodule/capsule formation to remove/encapsulate large bacterial aggregates/pathogens from the hemolymph,respectively.These processes are supported by the release of melanin precursors from crystal cells and likely by mobilizing nutrient reserves in newly circulating adipohemocytes,which could thus trophically support other hemocytes during the immune response.Finally,the regulation of the cell-mediated im-mune response by eicosanoids was investigated.

关 键 词：EICOSANOIDS encapsulation HEMOCYTES Hermetia illucens immunity PHAGOCYTOSIS 

分 类 号：Q96[生物学—昆虫学]