Effect of the rearing diet on gene expression of antimicrobial peptides in Hermetia illucens(Diptera:Stratiomyidae)  

作　　者：Valentina Candian Carlotta Savio Marco Meneguz Laura Gasco Rosemarie Tedeschi 

机构地区：[1]Dipartimento di Scienze Agrarie,Forestali eAlimentari(DISAFA),University of Torino,Grugliasco,Italy [2]NRAE,Micalis,GME,University of Paris Saclay,Jouy-en-Josas,France [3]Laboratory of Entomology,Wageningen University,Wageningen,The Netherlands [4]BEF Biosystems,Torino,Italy

出　　处：《Insect Science》2023年第4期933-946,共14页昆虫科学（英文版）

摘　　要：Insect proteins have been proposed for human and animal food production.Safeguarding the health status of insects in mass rearing allows to obtain high-quality products and to avoid severe economic losses due to entomopathogens.Therefore,new strategies for preserving insect health must be implemented.Modulation of the insect im-mune system through the diet is one such strategy.We evaluated gene expression of two antimicrobial peptides(one defensin and one cecropin)in Hermetia illucens(L.)(Diptera:Stratiomyidae)reared on different diets.Analyses were performed on prepupae and 10-day-old larvae reared on cereal-and municipal organic waste-based diets and on only prepupae reared on a cereal-based diet supplemented with sunflower,corn,or soybean oil.The inclusion of sunflower oil at different points in the cereal-based diet was also evalu-ated.Moreover,diet-driven differences in the inhibitory activity of the hemolymph were tested against Escherichia coli DH5αand Micrococcus yunnanensis HI55 using diffusion assays in solid media.Results showed that a municipal organic waste-based diet produced a significant overexpression of antimicrobial peptides only in prepupae.Inclusion of veg-etable oils caused an upregulation of at least one peptide,except for the corn oil.Higher expression of both genes was observed when sunflower oil was added 5 days before pupa-tion.All hemolymph samples showed an inhibitory activity against bacteria colonies.Our results suggest that municipal organic waste-based diet and vegetable oil-added diet may successfully impact the immune system of H.illucens.Such alternatives may also exist forotherspeciesofeconomic interest.

关 键 词：CECROPIN CEREAL DEFENSIN HEMOLYMPH organic municipal solid waste vegetable oil 

分 类 号：Q96[生物学—昆虫学]