Naringin Inhibits Colorectal Carcinogenesis by Inhibiting Viability of Colorectal Cancer Cells  

作　　者：ZENG Juan-ni TAN Jin-yu MO Li 

机构地区：[1]Department of Anus-Intestines, the Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha(410008),China

出　　处：《Chinese Journal of Integrative Medicine》2023年第8期707-713,共7页中国结合医学杂志（英文版）

基　　金：General Project of Natural Science Foundation of Hunan Province(No.2021JJ30518);Science and Technology Innovation Plan Project of Hunan Provincial(No.2021SK51302);Domestic First-Class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine。

摘　　要：Objective To explore the therapeutic effect of naringin on colorectal cancer(CRC)and the related mechanism.Methods Cell counting kit-8(CCK-8)assay and annexin V-FITC/PI assay were used to detect the effect of naringin(50–400µg/mL)on cell proliferation and apoptosis of CRC cells,respectively.The scratch wound assay and transwell migration assay were used to assess the effect of naringin on CRC cell migration.Four-week-old male nude mice were injected with HCT116 cells subcutaneously to establish the tumor xenograft model.Naringin was injected intraperitoneally at 50 mg/(kg·d),with solvent and 5-fluorouracil treatment as control.The width and length of the tumors were measured and recorded every 6 days,and tumor tissues were photographed and weighed on the last day of the 24-d observation period.Immunohistochemical staining for caspase-3,proliferating cell nuclear antigen and TUNEL assay were used to evaluate the effect of naringin on cell proliferation and apoptosis in tumor tissues.The body weight,food and water intake of mice were recorded,and the major organs in different treatment groups were weighed on the last day and stained with hematoxylin and eosin for histological analysis.Meanwhile,the routine blood indicators were recorded.Results CCK-8 and annexin V-FITC/PI results confirmed that naringin(100,200,and 400µg/mL)could inhibit proliferation and promote apoptosis.The scratch wound assay and transwell migration assay results confirmed the inhibitory activity of naringin against CRC cells migration.In vivo results demonstrated the inhibitory effect of naringin on tumor growth with good bio-compatibility.Conclusion Naringin inhibited colorectal carcinogenesis by inhibiting viability of CRC cells.

关 键 词：apoptosis colorectal cancer migration NARINGIN PROLIFERATION tumor 

分 类 号：R285.5[医药卫生—中药学]