驱动蛋白23调控骨肉瘤进展及血管新生  

Regulatory effect of kinesin family member 23 on progression of osteosarcoma and angiogenesis

作　　者：金池 卢新昌[1] 闻嘉[1] 刘永奎[1] 李甲振[1] 张翼[1] Jin Chi;Lu Xinchang;Wen Jia;Liu Yongkui;Li Jiazhen;Zhang Yi(Department of Orthopedics,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院骨科,郑州450052

出　　处：《中华实验外科杂志》2023年第6期1075-1078,共4页Chinese Journal of Experimental Surgery

基　　金：河南省高等学校重点科研项目(23A320047);3D打印聚醚醚酮(PEEK)材料复合缓释阿霉素在恶性骨肿瘤综合治疗中的应用研究(222102310245)。

摘　　要：目的:探究驱动蛋白家族成员23(KIF23)在骨肉瘤组织中的表达水平,在骨肉瘤中的调控方式。方法:收集2015年3月至2023年3月期间郑州大学第一附属医院30例骨肉瘤患者手术切除的肿瘤及癌旁组织标本,全部病例均经过病理证实,其中男18例,女12例,年龄(14.0±1.9)岁。利用KIF23特异性抗体检测其在人骨肉瘤组织及癌旁组织中的表达量,并利用KIF23特异的短发卡(shRNA)质粒在成骨肉瘤细胞(U2-OS)及人脐静脉血管内皮细胞(HUVEC)中下调KIF23的表达,通过噻唑蓝(MTT),细胞计数试剂盒(CCK-8)及划痕实验检测KIF23对内皮细胞增殖及迁移的影响。内皮细胞体外成管(Tube formation)实验检测对HUVEC体外成管的影响。蛋白质印迹法(Western blot)检测细胞增殖及迁移相关标志物的表达;定量聚合酶链反应(qPCR)检测相关mRNA的表达;裸鼠成瘤实验检测下调KIF23后裸鼠体内成瘤情况。结果:KIF23的mRNA水平在骨肉瘤组织中较对照组差异有统计学意义(P<0.01);U2-OS细胞中,细胞增殖和细胞迁移在转染组中较对照组差异有统计学意义(P<0.01);HUVEC中,转染组较对照组在细胞增殖和迁移差异有统计学意义(P<0.01);KIF23敲低组较对照组在肿瘤质量差异有统计学意义(P<0.01),体积差异也有统计学意义(P<0.01)。结论:KIF23调控体内外骨肉瘤细胞增殖迁移及内皮细胞增殖迁移,进一步调控肿瘤进展及血管新生过程。Objective To explore the expression level of kinesin family member 23(KIF23)in osteosarcoma tissue and its regulatory mode in osteosarcoma(OS).Methods The tumor and adjacent tissue samples were collected from 30 patients with osteosarcoma who underwent surgical resection at the First Affiliated Hospital of Zhengzhou University from March 2015 to March 2023.All cases were confirmed by pathology,including 18 male patients and 12 female patients,with an average age of(14.0±1.9)years.KIF23 specific antibody was used to detect the expression of KIF23 in human OS tissues,and KIF23 short hairpin RNA(shRNA)plasmids were transfected into(U2-OS)osteosarcoma cells and human umbilical vein endothelial cells(HUVECs),respectively,to deplete the expression of KIF23.Methyl thiazolyl tetrazolium(MTT),cell counting kit-8(CCK-8)and wound closure assays were performed to detect the effects of KIF23 on OS and endothelial cell proliferation and migration.The effects of KIF23 on HUVEC in vitro angiogenesis were further detected by the tube formation assays.Western blotting and quantitative polymerase chain reaction(qPCR)were used to detect the expression of cell proliferation and migration markers.Tumor formation in nude mice was detected after down-regulation of KIF23.Results The mRNA level of KIF23 in OS tissue showed a statistically significant difference compared to the control group(P<0.01).In OS cells,cell proliferation and cell migration in the transfection group were significantly different from those in the control group(P<0.01).In HUVECs,there was a statistically significant difference in cell proliferation and migration between the transfected group and the control group(P<0.01).There was statistically significant difference in tumor quality and volume between the KIF23 knockdown group and the control group(P<0.01).Conclusion KIF23 was highly expressed in OS tissues,promoted the proliferation and migration of OS and endothelial cells in vitro,and further regulated OS progression and angiogenesis.

关 键 词：驱动蛋白家族成员23 骨肉瘤 人脐静脉血管内皮细胞 增殖 迁移 血管新生 

分 类 号：R738.1[医药卫生—肿瘤]