细胞周期数依赖性蛋白激酶4和三结构域蛋白58在结直肠组织的表达及临床意义  

Clinical value of cell cycle number dependent protein kinase 4 and tripartite motif 58 expression in colorectal cancer tissues

作　　者：黄哲 胡旭 张丁月 金小琴 魏怡秋 钟俊 蔡朋株 Huang Zhe;Hu Xu;Zhang Dingyue;Jin Xiaoqin;Wei Yiqiu;Zhong Jun;Cai Pengzhu(Department of Anorectal Surgery,Affiliated Hospital of Guangdong Medical University,Zhanjiang 524001,China;Oncology Center,Affiliated Hospital of Guangdong Medical University,Zhanjiang 524001,China)

机构地区：[1]广东医科大学附属医院肛肠外科,湛江524001 [2]广东医科大学附属医院肿瘤中心,湛江524001

出　　处：《中华实验外科杂志》2023年第6期1131-1134,共4页Chinese Journal of Experimental Surgery

基　　金：2020年度广东省医学科研基金立项项目(B2020112);2021年度湛江市科技发展专项资金竞争性分配项目(2021A05080);2019年度广东医科大学附属医院院内资助类临床研究项目(LCYJ2019B007)。

摘　　要：目的:探讨细胞周期数依赖性蛋白激酶4(CDK4)和三结构域蛋白58(TRIM58)在结直肠癌组织中的表达变化,及其在结直肠癌发生发展中的作用和对患者预后的影响。方法:收集广东医科大学附属医院2020年3月至2022年11月病理确诊的117例结直肠癌组织标本和癌旁组织标本,采用免疫组织化学法检测以上组织中TRIP6和TRIM58表达,采用χ^(2)检验分析分析CDK4和TRIM58表达与结直肠癌临床病理因素及预后的相关性。结果:CDK4在结直肠癌组织中阳性表达率为63.25%(74/117),明显高于癌旁组织中阳性表达率35.04%(41/117),差异有统计学意义(χ^(2)=18.621,P<0.01)。CDK4表达水平与结直肠癌患者淋巴结转移、组织学分化程度、TNM分期(TNM stage)、浸润深度明显相关(χ^(2)=4.836、5.376、6.228、6.935,P<0.05),CDK4表达水平与结直肠癌患者性别、年龄、肿瘤部位无明显相关(χ^(2)=0.002、0.163、0.022,P>0.05)。TRIM58在结直肠癌组织中阳性表达率为33.33%(39/117),明显低于癌旁组织中阳性表达率65.81%(77/117),差异有统计学意义(χ^(2)=24.686,P<0.01)。TRIM58表达水平与结直肠癌患者淋巴结转移、组织学分化程度、TNM分期明显相关(χ^(2)=5.723、6.899、5.571,P<0.05),TRIM58表达水平与结直肠癌患者性别、年龄、浸润深度、肿瘤部位无明显相关(χ^(2)=0.017、0.154、0.026、0.287,P>0.05)。结论:结直肠癌组织中CDK4表达上调、TRIM58表达下调,对结直肠癌患者进行CDK4和TRIM58的检测可评估肿瘤的恶性程度并判断患者预后。Objective To investigate the expression changes of cell cycle number dependent protein kinase 4(CDK4)and tripartite motif 58(TRIM58)in colorectal cancer tissues,their role in the occurrence and development of colorectal cancer and their effect on the prognosis of patients with colorectal cancer.Methods Totally,117 cases of colorectal cancer tissues and adjacent normal tissues were collected at the Affiliated Hospital of Guangdong Medical University from March 2020 to November 2022.Immunohistochemistry was used to detect the expression of TRIP6 and TRIM58 in the above tissues.Chi-square test was used to analyze the correlation between CDK4 and TRIM58 expression and clinicopathological factors and prognosis of colorectal cancer.Results The positive expression rate of CDK4 in colorectal cancer tissues was 63.25%(74/117),which was significantly higher than that in adjacent normal tissues[35.04%(41/117),χ^(2)=18.621,P<0.01].The expression level of CDK4 was significantly correlated with lymph node metastasis,histological differentiation,tumor node metastasis stage(TNM stage)and depth of invasion in patients with colorectal cancer(respectively,χ^(2)=4.836,5.376,6.228,6.935,P<0.05).The expression level of CDK4 was not significantly correlated with gender,age and tumor location of colorectal cancer patients(respectively,χ^(2)=0.002,0.163,0.022,P>0.05).The positive expression rate of TRIM58 in colorectal cancer tissues was 33.33%(39/117),which was significantly lower than 65.81%(77/117)in adjacent normal tissues(χ^(2)=24.686,P<0.01).The expression level of TRIM58 was significantly correlated with lymph node metastasis,histological differentiation and TNM stage of colorectal cancer patients(respectively,χ^(2)=5.723,6.899,5.571,P<0.05).The expression level of TRIM58 was not significantly correlated with gender,age,depth of invasion and tumor location of colorectal cancer patients(respectively,χ^(2)=0.017,0.154,0.026,0.287,P>0.05).Conclusion CDK4 expression is up-regulated and TRIM58 expression is down-regulated in colo

关 键 词：细胞周期数依赖性蛋白激酶4 结直肠癌 三结构域蛋白58 免疫组织化学方法 

分 类 号：R735.34[医药卫生—肿瘤]