Hydrogels for bone organoid construction:From a materiobiological perspective  

作　　者：Shunli Wu Xianmin Wu Xiuhui Wang Jiacan Su 

机构地区：[1]Institute of Translational Medicine,Shanghai University,Shanghai 200444,China [2]School of Medicine,Shanghai University,Shanghai 200444,China [3]School of Environmental and Chemical Engineering,Shanghai University,Shanghai 200444,China [4]Department of Orthopedics Trauma,Zhongye Hospital of Shanghai,Shanghai 201900,China [5]Department of Orthopaedics Trauma,Changhai Hospital,Second Military Medical University,Shanghai 200433,China

出　　处：《Journal of Materials Science & Technology》2023年第5期21-31,共11页材料科学技术（英文版）

基　　金：This work was funded by the National Key R&D Program of China(No.2018YFC2001500);the National Natural Science Foun-dation of China(Nos.82172098,82001968);Shanghai Pujiang Program(No.20PJ1403800).

摘　　要：Bone organoids,which simulate and construct special organs in vitro with complex biological func-tions based on tissue engineering technology,provide dramatically realistic models for bone regenerative medicine development and lay the foundation for a new therapeutic strategy.The matrix microenviron-ment around tissues and cells plays a key role in the physiological functions and phenotypes of bone organoids.Traditionally,the commercially available Matrigel has been widely applied for organoid cul-tures.However,Matrigel is still facing challenges,including xenogenous origins and variable composi-tion.To address these issues,newly developed hydrogels become an appropriate candidate to alternate Matrigel for bone organoid culture.In this review,we summarized the development and limitations of ECM-based matrix(Matrigel)in the bone organoid cultures.Then we highlighted various hydrogel al-ternatives,including PEG,collagen,alginate,gelatin,chitosan,skin fibroin,and DNA derivative hydrogels,which have shown a promising application in bone tissue engineering and organoid cultures.Additionally,the effects of material properties(stiffness,viscoelasticity,charge,et al.)in hydrogels on cell culture and bone organoid culture were deeply investigated.Finally,we predicted that hydrogel-based biomaterials have a great potential for the construction and application of bone organoids.

关 键 词：HYDROGELS Bone organoid MATRIGEL Extracellular matrix Bone tissue engineering 

分 类 号：R738.1[医药卫生—肿瘤] TQ427.26[医药卫生—临床医学]