从脑肠轴探讨黄芪建中汤对胃溃疡大鼠肝细胞生长因子及ERK1/2和TFF3蛋白表达的影响  

Effect of Huangqi Jianzhong Decoction on the expression of hepatocyte growth factor and ERK1/2 and TFF3 proteins in rats with gastric ulcer from brain-gut axis perspectives

作　　者：陈思清 韩运宗 刘琴[1] 周姝 周赛男[2] CHEN Siqing;HAN Yunzong;LIU Qin;ZHOU Shu;ZHOU Sainan(Hunan University of Traditional Chinese Medicine,Changsha 410007,Hunan,China;The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410021,Hunan,China)

机构地区：[1]湖南中医药大学,湖南长沙410007 [2]湖南中医药大学第一附属医院,湖南长沙410021

出　　处：《现代中西医结合杂志》2023年第12期1651-1655,1728,共6页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：湖南中医药大学中医学国内一流建设学科项目(2018ZYX13);湖南省中医药科研计划重点项目(2021203);湖南省自然科学基金科卫联合项目(2022JJ70031)。

摘　　要：目的观察黄芪建中汤对脾胃虚寒型胃溃疡大鼠胃组织中肝细胞生长因子(HGF)及下丘脑和海马区中磷酸化细胞外信号调节激酶1/2(p-ERK1/2)及肠三叶因子3(TFF3)蛋白表达的影响,探究黄芪建中汤治疗脾胃虚寒型胃溃疡的作用及可能机制。方法用随机数字表法将60只大鼠分为正常组、模型组、奥美拉唑组和黄芪建中汤组,每组15只。正常组隔日蒸馏水灌胃,每日不限饮食;其余组大鼠先以小承气汤结合饥饱失常法复制脾胃虚寒证模型,实验第11天采用冰醋酸法建立胃溃疡模型。之后模型组继续隔日上午给予小承气汤并当日禁食,次日恢复饮食,共持续20 d;奥美拉唑组和黄芪建中汤组大鼠除同模型组的每日处理外,每日下午分别给予4.2 mg/(kg·d)奥美拉唑和6.8 g/(kg·d)黄芪建中汤灌胃;正常组隔日上午及每日下午给予蒸馏水灌胃。实验结束后摘取各组大鼠胃,记录溃疡大小并计算胃溃疡指数,HE染色观察胃组织病理形态,免疫组化染色检测胃组织中HGF及下丘脑和海马区中p-ERK1/2、TFF3蛋白阳性表达情况。结果正常组大鼠胃黏膜正常,未见溃疡点及糜烂斑等;模型组大鼠胃黏膜皱襞存在中断,有点状溃疡、糜烂及大量炎性细胞浸润;黄芪建中汤组与奥美拉唑组胃黏膜损伤较模型组轻,有少量炎性细胞浸润,未见明显溃疡。奥美拉唑组和黄芪建中汤组大鼠的胃溃疡指数均明显低于模型组(P均<0.05),胃组织中HGF及下丘脑和海马区中p-ERK1/2、TFF3蛋白表达平均光密度均明显高于模型组(P均<0.05)。结论黄芪建中汤能促进脾胃虚寒型胃溃疡大鼠溃疡愈合,上调HGF、ERK1/2及TFF3的表达可能是其基于脑肠轴治疗脾胃虚寒型胃溃疡的作用机制之一。Objective It is to observe the effect of Huangqi Jianzhong Decoction on the expression of hepatocyte growth factor(HGF)in gastric mucosa and the expression of phosphorylated extracellular signal-regulated kinase 1/2(ERK1/2)and intestinal trefoil factor 3(TFF3)in hypothalamus and hippocampus of rats with gastric ulcer of type of spleen-stomach deficiency cold,to explore the effect and possible mechanism of this decoction in the treatment.Methods Sixty rats were divided into normal group,model group,omeprazole group and Huangqi Jianzhong Decoction group by random number table method,with 15 rats in each group.The rats in the normal group were gavaged with distilled water every other day,and no dietary restrictions were imposed daily;the rats in the remaining groups were first given Xiao-Cheng-Qi Decoction combined with irregular diet to replicate the models of syndrome of spleen and stomach deficiency syndrome,and the gastric ulcer models were established by the glacial acetic acid method on the 11th day of the experiment.After that,the model group was continuously given Xiao-Cheng-Qi Decoction every other morning and fasted on the same day,and resumed diet on the next day,totally treated for 20 days.In addition to the same daily treatment as that of the model group,the rats in the omeprazole group and Huangqi Jianzhong Decoction group were given omeprazole 4.2 mg/(kg·d)and Huangqi Jianzhong Decoction 6.8 g/(kg·d)by gavage in the afternoon of each day,respectively;the rats in the normal group were given distilled water by gavage in the morning of every other day and in the afternoon of each day.At the end of the experiment,the stomachs of the rats in each group were extracted,the ulcer size was recorded and the gastric ulcer index was calculated,the pathological morphology of gastric tissues was observed by HE staining,and the positive expression of HGF in gastric tissues and p-ERK1/2 and TFF3 proteins in the hypothalamus and hippocampus areas were detected by immunohistochemical staining.Results The gastric muco

关 键 词：胃溃疡 脑肠轴 脾胃虚寒证 黄芪建中汤 肝细胞生长因子 细胞外信号调节激酶1/2 肠三叶因子3 

分 类 号：R-332[医药卫生]