乐伐替尼对甲状腺癌SW579细胞放射敏感性及血管内皮生长因子、碱性成纤维细胞生长因子、碱性成纤维细胞生长因子受体表达的影响  

Effects of lenvatinib on radiosensitivity and expression of VEGF,bFGF and bFGFR in thyroid cancer SW579 cells

作　　者：赵莉娅 赵铁铮[2] 庞英 杨娜 范彦军 ZHAO Liya;ZHAO Tiezheng;PANG Ying;YANG Na;FAN Yanjun(Special Service Brigade of Shijiazhuang Emergency Center,Shijiazhuang 050000,China)

机构地区：[1]石家庄市急救中心特勤大队,河北石家庄050000 [2]黄骅市人民医院普外科,河北黄骅061100

出　　处：《陕西医学杂志》2023年第8期956-959,968,共5页Shaanxi Medical Journal

基　　金：河北省医学科学研究计划项目(20210533)。

摘　　要：目的:探究乐伐替尼对甲状腺癌SW579细胞放射敏感性以及对血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)及其受体(bFGFR)表达的影响。方法:体外培养甲状腺癌SW579细胞,筛选最佳放射剂量,取培养至对数生长期的SW579细胞分为对照组(常规培养SW579细胞)、放射组(使用4 Gy X射线照射SW579细胞)、乐伐替尼低(在含SW579细胞的培养基中加入5μmol/L的乐伐替尼后,再使用4 Gy X射线照射SW579细胞)、高(在含SW579细胞的培养基中加入10μmol/L的乐伐替尼后,再使用4 Gy X射线照射SW579细胞)浓度组,培养48 h后。检测SW579细胞存活率、细胞凋亡率和细胞中B细胞淋巴瘤-2相关X(Bax)、B细胞淋巴瘤-2(Bcl-2)、活化胱天蛋白酶3(Cleaved caspase-3)、VEGF、bFGF、bFGFR蛋白表达。结果:随着放射剂量的升高,SW579细胞存活率依次降低(均P<0.05),选取4 Gy X射线进行后续实验;与对照组相比,放射组、乐伐替尼低、高浓度组SW579细胞存活率、Bcl-2、VEGF、bFGF、bFGFR蛋白表达依次降低(均P<0.05),细胞凋亡率、Bax、Cleaved caspase-3蛋白表达依次升高(均P<0.05)。结论:乐伐替尼能增强SW579细胞的放射敏感性,其机制可能与抑制VEGF、bFGF、bFGFR蛋白表达有关。Objective:To investigate the effects of lenvatinib on the radiosensitivity and the expression of vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF)and its receptor(bFGFR)in thyroid cancer SW579 cells.Methods:Thyroid cancer SW579 cells were cultured in vitro to screen the optimal dose of radiation.SW579 cells cultured to logarithmic growth stage were divided into control group(conventional culture SW579 cells),radiation group(SW579 cells were irradiated with 4 Gy X-ray),levastinib low concentration group(after adding 5μmol/L of levatinib to the medium containing SW579 cells,SW579 cells were irradiated with 4 Gy X-ray)and levastinib high concentration group(after adding 10μmol/L of levatinib to the medium containing SW579 cells,SW579 cells were irradiated with 4 Gy X-ray),and cultured for 48 hours.Cell survival rate,apoptosis rate,the protein expressions of B-cell lymphoma-2-associated X protein(Bax),B-cell lymphoma-2(Bcl-2),activated Caspase-3(Cleaved caspase-3),VEGF,bFGF,and bFGFR in SW579 were detected.Results:With the increase of radiation dose,the survival rate of SW579 cells decreased in turn(all P<0.05),and 4 Gy X-ray was selected for follow-up experiments.Compared with control group,survival rate,protein expressions of Bcl-2,VEGF,bFGF and bFGFR of SW579 cells in radiation group,lovastinib low concentration group and high concentration group were decreased in turn,and the apoptosis rate,protein expressions of Bax,Cleaved caspase-3 were increased in turn(all P<0.05).Conclusion:Lenvatinib can enhance the radiosensitivity of SW579 cells,and the mechanism may be related to the inhibition of VEGF,bFGF and bFGFR protein expressions.

关 键 词：乐伐替尼 甲状腺癌SW579细胞 血管内皮生长因子 碱性成纤维细胞生长因子 碱性成纤维细胞生长因子受体 放射敏感性 

分 类 号：R736.1[医药卫生—肿瘤]