微小核糖核酸34a与沉默信息调节因子1在宫颈癌组织中的表达及相关性研究  

Expression and association analysis of miR-34a and SIRT1 in cervical cancer tissues

作　　者：陈庆[1] 李乙航 赵金燕[1] 吴晓玲[1] 谢秀英[1] CHEN Qing;LI Yihang;ZHAO Jinyan;WU Xiaoling;XIE Xiuying(The Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,China)

机构地区：[1]西安交通大学第二附属医院,陕西西安710004 [2]长春生物制品研究所有限责任公司,吉林长春130012

出　　处：《陕西医学杂志》2023年第8期1089-1092,1105,共5页Shaanxi Medical Journal

基　　金：陕西省重点研发计划项目(2023YBSF217);陕西省自然科学基金资助项目(2017JM8153)。

摘　　要：目的:探讨微小核糖核酸34a(miR-34a)及沉默信息调节因子1 (SIRT1)在宫颈癌组织中的表达及其相关性。方法:收集住院患者标本,采用RT-qPCR法检测miR-34a在正常宫颈、宫颈癌组织中的表达;免疫组化法检测SIRT1在宫颈癌组织及正常宫颈组织中的表达,分析miR-34a及SIRT1表达与宫颈癌患者临床病理参数的关系,并作Spearman相关分析。结果:miR-34a在宫颈癌组织中的表达明显低于正常宫颈组织(P<0.05),且miR-34a在宫颈癌组织的表达与肿瘤分化程度显著相关(均P<0.05);免疫组化显示SIRT1在宫颈癌组织中的表达高于正常宫颈组织,其阳性表达率分别为84%与20%,差异具有统计学意义(P<0.05);Spearman分析显示,SIRT1与miR-34a在宫颈癌组织中的表达呈负相关(r=-0.327,P<0.05)。结论:miR-34a在宫颈癌的发生中起重要作用,这为宫颈癌临床治疗提供了一个潜在靶点。Objective:To investigate the expression of and association of microRNA-34a(miR-34a)and silent information regulator 1(SIRT1)in cervical cancer tissues.Methods:Samples of inpatients were collected,and the expression of miR-34a in normal cervix and cervical cancer tissues was detected by RT-qPCR.The expression of SIRT1 in cervical cancer tissues was detected by immunohistochemistry,and the relationship between the expression of miR-34a,SIRT1 and clinicopathologic parameters of patients with cervical cancer was analyzed.Spearman correlation analysis was performed.Results:The expression of miR-34a in cervical cancer tissues was significantly lower than that in normal cervix(P<0.05).The expression of miR-34a in cervical cancer tissues was significantly correlated with the degree of tumor differentiation(P<0.05).Immunohistochemistry showed that the expression of SIRT1 in cervical cancer tissues was higher than that in normal cervix(P<0.05),and the positive expression rates were 84%and 20%,respectively.Spearman analysis showed that SIRT1 was negatively correlated with miR-34a expression in cervical cancer tissues(r=-0.327,P<0.05).Conclusion:MiR-34a plays an important role in the development of cervical cancer,which provides a potential target for clinical treatment of cervical cancer.

关 键 词：微小核糖核酸34a 沉默信息调节因子1 宫颈癌 相关性 荧光定量PCR 免疫组化 

分 类 号：R737.33[医药卫生—肿瘤]