烟酰胺磷酸核糖基转移酶及沉默信息调节因子2同源蛋白1在乳腺癌组织中的表达及与患者临床病理特征的关系  

Expression of NAMPT and SIRT-1 in breast cancer tissues and their relationship with clinicopathologic features of patients

作　　者：邹腾跃 邹芳芳 孙亚君 尹俊倩 毕铁强 ZOU Tengyue;ZOU Fangfang;SUN Yajun;YIN Junqian;BI Tieqiang(School of Clinical Medicine,Weifang Medical College,Weifang 261053,China)

机构地区：[1]潍坊医学院临床医学院,山东潍坊261053 [2]山东大学附属威海市立医院,山东威海264200

出　　处：《陕西医学杂志》2023年第8期1093-1096,1101,共5页Shaanxi Medical Journal

基　　金：国家自然科学基金资助项目(81602319);江苏省连云港市科技计划项目(SH1329)。

摘　　要：目的:探讨在乳腺癌组织中烟酰胺磷酸核糖基转移酶(NAMPT)及沉默信息调节因子2同源蛋白1(SIRT-1)的表达率及与患者临床病理特征的关系。方法:选取手术成功切除乳房的381例乳腺癌患者为研究对象,均随访5年或随访至患者死亡,采用免疫组织化学方法检测三阴性乳腺癌患者、非三阴性乳腺癌患者NAMPT及SIRT-1的蛋白表达,并分析其与临床病特征的关系及NAMPT表达与SIRT-1表达的关系,分析NAMPT、SIRT-1表达与临床特征之间的相关性。结果:三阴性乳腺癌患者NAMPT高表达率高于非三阴性乳腺癌患者(P<0.05);年龄≥56岁、有淋巴结转移、无病生存期<55个月的乳腺癌患者NAMPT高表达率高于年龄<56岁、无淋巴结转移、无病生存期≥55个月的乳腺癌患者(均P<0.05);有淋巴结转移、无病生存期<55个月的乳腺癌患者SIRT-1高表达率高于年龄<56岁、无病生存期≥55个月的乳腺癌患者(均P<0.05);NAMPT高表达的乳腺癌患者SIRT-1高表达率高于SIRT-1低表达率(P<0.05),乳腺癌患者NAMPT、SIRT-1表达与淋巴结转移、无病生存期呈正相关(r=0.079、0.505;0.462、0.497,P<0.05)。结论:NAMPT在三阴性乳腺癌中表达上调,NAMPT可影响SIRT-1的表达,同时年龄可影响NAMPT及SIRT-1的表达,且两者高表达预示患者可能出现淋巴结转移、预后差。Objective:To investigate the expression rates of nicotinamide phosphoribosyltransferase(NAMPT)and silencing information regulator 2 homologous protein 1(SIRT-1)in breast cancer and their relationship with clinicopathologic features of patients.Methods:A total of 381 patients with breast cancer who were successfully removed by surgery were selected as subjects.All patients were followed up for 5 years or until death.Immunohistochemical methods were used to detect the protein expressions of NAMPT and SIRT-1 in triple negative breast cancer patients and non-triple negative breast cancer patients,and the relationship between the expressions of NAMPT,SIRT-1 and the clinical characteristics,and the relationship between the expressions of NAMPT and SIRT-1 were analyzed.Results:The high expression rate of NAMPT in triple negative breast cancer patients was higher than that in non-triple negative breast cancer patients(P<0.05).The high expression rate of NAMPT in breast cancer patients aged≥56 years with lymph node metastasis and disease-free survival time<55 months was higher than that in breast cancer patients aged<56 years without lymph node metastasis and disease-free survival time≥55 months(P<0.05).The high expression rate of SIRT-1 in breast cancer patients with lymph node metastasis and disease-free survival<55 months was higher than that in breast cancer patients with age<56 years and disease-free survival≥55 months(P<0.05).The high expression rate of SIRT-1 in breast cancer patients with high expression of NAMPT was higher than that with low expression of SIRT-1(P<0.05).The expressions of NAMPT and SIRT-1 in breast cancer patients were positively correlated with lymph node metastasis and disease-free survival(r=0.079,0.505,0.462,0.497,all P<0.05).Conclusion:The expression of NAMPT in triple negative breast cancer was obviously up-regulated,which can affect the expression of SIRT-1,and age might affect the expression of NAMPT and SIRT-1,and the high expression of both may indicate that the patient may have l

关 键 词：乳腺癌 烟酰胺磷酸核糖基转移酶 沉默信息调节因子2同源蛋白1 组织芯片技术 淋巴结转移 预后 

分 类 号：R737.9[医药卫生—肿瘤]