3D printed porous titanium filled with mineralized UV-responsive chitosan hydrogel promotes cell proliferation and osteogenesis in vitro  

作　　者：Jiazhao Yang Fan Liu Changshun Zhou Hejie Li Gaolin Yang Shiyuan Fang In-Seop Lee Yi Liu Hao Bai Cen Chen 

机构地区：[1]The First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230001,China [2]Department of Orthodontics,School of Stomatology,China Medical University,Shenyang 110002,China [3]College of Life Sciences and Medicine,Zhejiang Sci-Tech University,Hangzhou 310018,China [4]Institute of Laser Advanced Manufacturing,Zhejiang University of Technology,Hangzhou 310023,China [5]Institute of Human Materials,Suwon 16514,Republic of Korea [6]State Key Laboratory of Chemical Engineering,College of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310027,China [7]Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine,Hangzhou 310018,China

出　　处：《Journal of Materials Science & Technology》2023年第11期34-44,共11页材料科学技术（英文版）

基　　金：financially supported partly by the Zhejiang Provincial Natural Science Foundation of China(No.LY20E010006);partly by the Fundamental Research Funds for the Central Universities(No.WK9110000152);partly by the Key Research and Development Plan of Anhui Province(No.20194a0720097);partly by the National Natural Science Foundation of China(Nos.51502265 and 81701033).

摘　　要：The modification of 3D printed porous titanium(Ti),especially for the internal pore structure,is critical and has received more attention to promoting its osteogenesis for clinical use.Ultra-violet(UV)responsive chitosan(CSMA),as an injectable filling material,was firstly incorporated into porous Ti,and then CSMA was in-situ mineralized by carbon oxide(CO_(2))diffusion(CSMA/CaCO_(3)).Their physical-chemical and biological properties were investigated in vitro.CaCO_(3) crystals within CSMA hydrogels were successfully deposited into pores of porous Ti,which exhibited favorable biocompatibility.Ti implants filled with CSMA/CaCO_(3) promoted adhesion and proliferation of bone mesenchymal stem cells(BMSCs).Moreover,Ti implant filled CSMA/CaCO_(3) hydrogels could increase alkaline phosphatase(ALP)activities,up-regulate osteopontin(OPN)and osteocalcin(OCN)expression levels,and enhance extracellular mineralization.3D printed porous Ti filled with mineralized UV-responsive chitosan hydrogel could promote proliferation and osteogenesis of BMSCs,and have great potential for the modification of porous Ti implants in bone tissue engineering.

关 键 词：Porous titanium Chitosan Ultraviolet(UV)-responsive MINERALIZATION OSTEOGENESIS 

分 类 号：R318.08[医药卫生—生物医学工程] TG146.23[医药卫生—基础医学] TP391.73[一般工业技术—材料科学与工程]