磷酸盐与生物素最适浓度配比对谷氨酸发酵影响的研究  

Study on the Effect of Optimum Concentration Ratio of Phosphate to Biotin on Glutamic Acid Fermentation

作　　者：王锐麒 王硕 徐庆阳[1,2,3] WANG Rui-qi;WANG Shuo;XU Qing-yang(College of Bioengineering,Tianjin University of Science and Technology,Tianjin 300457,China;Tianjin Engineering Laboratory of Efficient and Green Amino Acid Manufacturing,Tianjin 300457,China;National and Local United Engineering Laboratory of Metabolic Control Fermentation Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]天津市氨基酸高效绿色制造工程实验室,天津300457 [3]代谢控制发酵技术国家地方联合工程实验室,天津300457

出　　处：《中国调味品》2023年第8期39-44,共6页China Condiment

基　　金：内蒙古自治区科技计划项目(2021GG0299);呼伦贝尔市“科技兴市”重点专项(2021hzzx07)。

摘　　要：在L-谷氨酸发酵中,磷酸盐和生物素的添加量直接影响着谷氨酸棒状杆菌的菌体生长和菌体转型,生物素和磷酸盐的含量过多或过少都会直接影响L-谷氨酸的产量,为缓解这一问题,提出了调配磷酸盐与生物素的最适浓度配比(PV H)的策略控制发酵。为了确定发酵过程中生物素和磷酸盐的准确含量,利用单因素实验对磷酸盐与生物素的浓度配比进行调整,以找出最有利于菌体快速生长和细胞迅速转型的实验组。该实验设计生物素浓度梯度为4,10,14μg/L,磷酸盐浓度梯度为3,5 g/L,通过实验发现,当磷酸盐与生物素浓度配比为5∶14时,菌体量大,细胞转型快,产酸多,糖酸转化率高,在此条件下,最大OD 600 nm达到82,较其他组分别提高了24%、47%、50%、65%,最终的菌体量为74,较其他组分别提高了29.7%、48.6%、54%、72%,L-谷氨酸的产量为86 g/L,相较于其他组分别提高了30%、45.3%、54.6%、80%。同时,在最适PV H条件下常规发酵L-谷氨酸的实验中可以看出,其整体发酵水平高于其他非最适PV H组,该常规发酵实验中,L-谷氨酸产量高达178 g/L,转化率达到77%,对于谷氨酸发酵具有借鉴意义。In the fermentation of L-glutamic acid,the addition amount of phosphate and biotin directly affects the bacterium growth and transformation of Corynebacterium glutamicum.Too much or too little biotin and phosphate will directly affect the yield of L-glutamic acid.In order to alleviate this problem,the optimal concentration ratio of phosphate to biotin(PV H)strategy is put forward to control the fermentation.In order to determine the accurate content of biotin and phosphate in the fermentation process,the concentration ratio of phosphate to biotin is adjusted by single factor experiment,so as to find out the experimental group which is most beneficial to the rapid growth of bacteria and the rapid transformation of cells.In this experiment,the concentration gradient of biotin is 4,10,14μg/L,and the concentration gradient of phosphate is 3,5 g/L.Through the experiment,it is found that when the concentration ratio of phosphate to biotin is 5∶14,the bacterial volume is large,cell transformation is fast,acid production is high,and sugar-acid conversion rate is high.Under such conditions,the maximum OD 600 nm reaches 82,which is 24%,47%,50%,65%higher than that of the other groups respectively. The final bacterial volume is 74, which is 29.7%, 48.6%, 54%, 72% higher than that of the other groups respectively. The L-glutamate yield is 86 g/L, which is 30%, 45.3%, 54.6%, 80% higher than that of the other groups respectively. At the same time, in the experiment of conventional fermentation of L-glutamic acid under optimal PV H conditions, it can be seen that the overall fermentation level is higher than that of other non-optimal PV H groups. In this conventional fermentation experiment, the yield of L-glutamic acid is as high as 178 g/L and the conversion rate is as high as 77%, which has reference significance for glutamic acid fermentation.

关 键 词：L-谷氨酸 生物素 磷酸盐 糖酸转化率 磷酸盐与生物素浓度配比 常规发酵 

分 类 号：TS201.24[轻工技术与工程—食品科学]