香橼与佛手F_(1)杂种鉴定及果形差异分析  

Identification of F_(1) Hybrids and Analysis of Fruit Shape Difference Between Fingered Citron and Citron

作　　者：梅煜琳 徐劲剑 蒋梦玥 尉俊海 宗宇[1] 陈文荣[1] 廖芳蕾[1,2] 郭卫东 MEI Yulin;XU Jinjian;JIANG Mengyue;YU Junhai;ZONG Yu;CHEN Wenrong;LIAO Fanglei;GUO Weidong(College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua,Zhejiang 321004,China;Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants,Jinhua,Zhejiang 321004,China)

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]浙江省特色经济植物生物技术研究重点实验室,浙江金华321004

出　　处：《园艺学报》2023年第7期1402-1418,共17页Acta Horticulturae Sinica

基　　金：金华市科学技术研究计划项目(2020-2-006)。

摘　　要：以香橼(Citrus medica L.)为母本,佛手(C.medica var.L.sarcodactylis Swingle)为父本进行授粉杂交,将得到的种子进行培育获得19份材料。从佛手和香橼转录组数据筛选出SSR位点设计TP-M13-SSR分子标记引物,对19份材料进行基因分型。每对核心引物获得17~32个等位基因,平均等位基因数为24.188个。观测杂合度变化为0.188~1.000,多态性指数为0.944。19个F_(1)代株系中17个确定为真杂种,杂交率为89.47%。通过影像分析F_(1)代果形,指状果与柑果果形分离比为2︰3。佛手和香橼花芽石蜡切片观察发现,形成指状或柑果果形差异起始于现蕾期至现瓣期的心皮隆起。对现蕾期至花后3d的佛手、香橼花芽转录组信息与心皮特征进行相关性分析,分离得到一批雌蕊形成相关基因;在即将开花的佛手、香橼及F_(1)代雌蕊中进行qRT-PCR验证,分析这些基因与果形差异的关联性,WOX1基因在现蕾期和现瓣期的表达与指状特征相关。YABBY家族基因可与花发育C类和E类基因及WOX家族协同作用调控佛手指状果形建成。In this study,19 F_(1) hybrids derived from the cross between Citrus medica L.and C.medica var.L.sarcodactylis Swingle were cultured as materials.SSR loci were selected from the transcriptome data of fingered citron and citron.Based on this,TP-M13-SSR primers were designed and then 19 F_(1) hybrids were genotyped.A total of 17 to 32 alleles were obtained from 16 SSR primers,and the average number of alleles was 24.188.The heterozygosity was ranged from 0.188 to 1.000,and the polymorphism index was about 0.944.The results showed that 17 out of 19 F_(1) lines were true hybrids,and the hybridization rate was 89.47%.Those materials were phenotyped by morphological photography analysis and the ratio of finger fruit to hesperidium is 2︰3.Paraffin section analysis of early flower buds of fingered citron and citron found the difference between their fruit shape initiating from the carpel bulges since the visible bud stage to the petals emerging stage.From the visible bud stage to 3 d after anthesis of fingered citron and citron,the correlation analysis between the transcriptome data and carpel feature was done,and a number of genes associated with gynoecium formation were identified.These genes were validated in the pistils of citron,fingered citron,and hybrid F_(1) lines during the pre-anthesis stage.The results showed that the expression of WOX1 was related to the character of finger fruit from the visible bud stage to the petals emerging stage.The YABBY family coordinated with the classes C,E genes of the ABC model of flower development and WOX family to regulate the fruit shaping in fingered citron.

关 键 词：香橼 佛手 SSR 指状果 雌蕊形成 WOX1 

分 类 号：S666[农业科学—果树学]