Ercao Qinggan decoction regulates apoptosis of hepatocytes in mice with acute liver failure via the TLR4-mediated PI3K/Akt/GSK3βsignal pathway  

作　　者：Qin Ouyang Chun-Ming Wu San-Hai Liu Li-Jia Wang Jia-Min Yao An-Jing Zhao Jin-Lian Zheng Jian-Ying Huang 

机构地区：[1]Department of Infection,Wenzhou Hospital of Traditional Chinese Medicine,Wenzhou 325000,China

出　　处：《TMR Integrative Medicine》2023年第20期1-9,共9页TMR整合医学

基　　金：The National Natural Science Foundation of Zhejiang Provincial(No.LGF21H270001);the Association of Integrated Chinese and Western Medicine Research Fund Project of Zhejiang Provincial(No.2019LY013);the Basic Research Project of Wenzhou Science and Technology Bureau(No.Y20210149)supported this study.

摘　　要：Background:To clarify the inhibitory effect of Ercao Qinggan decoction(EQD)on acute liver failure(ALF)and its related mechanisms.Methods:HL-7702 hepatocytes were pretreated with TLR4 inhibitor CLI-095,glycogen synthase kinase 3β(GSK3β)inhibitor LiCl and different doses of EQD for 2 hours,and lipopolysaccharide(LPS)(10μg/mL)for 24 hours.Cell apoptosis,TNF-αand IL-6 and GSK3βwere detected by flow cytometry,immunofluorescence,quantitative polymerase chain reaction.After mice were gavaged with different concentrations of EQD for 12 days,ALF mouse models were established intraperitoneal injection of D-Gal/LPS.After 24 hours,the mice were euthanized and the liver tissue was stained with hematoxylin and eosin.Liver cell apoptosis,the serum levels of aspartate aminotransferase,alanine aminotransferase,TNF-αand IL-βwere detected by terminal transferase-mediated dUTP nick end-labelling,enzyme linked immunosorbent assay,quantitative polymerase chain reaction,and Western blotting,respectively.These methods were also used to test the mRNA expression of Bax,Bcl-2 and the protein expression of GSK3β,p-Akt/Akt in livers.Results:CLI-095,LiCl,and EQD significantly inhibited apoptosis induced by LPS,the mRNA expression of IL-6,TNF-αand the nuclear translocation of GSK3βin HL-7702 hepatocytes.EQD dose-dependently inhibited hepatocyte apoptosis,the serum concentration of aspartate aminotransferase and ALT,the expression of TNF-αand IL-β,the ratio of p-GSK3β/GSK3β,p-Akt/Akt in alanine aminotransferase mice.Conclusion:EQD can inhibit hepatocyte apoptosis in ALF mice through regulating TLR4/PI3K/Akt/GSK3βsignaling pathway.

关 键 词：acute liver failure Ercao Qinggan decoction LIPOPOLYSACCHARIDE HL-7702 TLR4 

分 类 号：R575.3[医药卫生—消化系统]