LINC01268 promotes epithelial-mesenchymal transition,invasion and metastasis of gastric cancer via the PI3K/Akt signaling pathway and targeting MARCKS  

作　　者：Ling-Han Tang Peng-Cheng Ye Lin Yao Ya-Jun Luo Wang Tan Wan-Ping Xiang Zi-Lin Liu Ling Tan Jiang-Wei Xiao 

机构地区：[1]Department of Gastrointestinal Surgery,Clinical Medical College and the First Affiliated Hospital of Chengdu Medical College,Chengdu 610000,Sichuan Province,China [2]Department of Gastrointestinal Surgery,The Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,Sichuan Province,China [3]Department of Gastrointestinal Surgery,Sichuan Cancer Hospital,Chengdu 610000,Sichuan Province,China [4]Department of Gastrointestinal Surgery,Yaan People’s Hospital,Yaan 625000,Sichuan Province,China [5]Department of Thoracic Surgery,The Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,Sichuan Province,China [6]Department of Surgery,People’s Hospital Affiliated to Chongqing Three Gorges Medical College,Chongqing 404041,China

出　　处：《World Journal of Gastrointestinal Oncology》2023年第8期1366-1383,共18页世界胃肠肿瘤学杂志（英文版）（电子版）

基　　金：Supported by the National Natural Science Foundation of China,No.81070378 and 81270561;Natural Science Foundation of Sichuan Province,China,No.2022NSFSC0050 and 2023NSFSC1896.

摘　　要：BACKGROUND Long non-coding RNAs(lncRNAs)with differential expression characteristics have been found to be closely related to the tumorigenesis and development of gastric cancer(GC),but their specific mechanisms and roles still need to be further elucidated.AIM To investigate the expression of LINC01268 in GC and its mechanism of affecting GC progression.METHODS Real-time quantitative polymerase chain reaction was used to detect the expression of LINC01268 in GC tissues,cell lines and plasma.The Kaplan-Meier method was used to evaluate the value of LINC01268 in the prognostication of GC patients.An receiver operating characteristic curve was constructed to evaluate the value of LINC01268 in the diagnosis of GC.Transwell migration and invasion assays and wound healing assays were used to confirm the effect of LINC01268 on the invasion and migration of GC cells.The regulatory relationship between LINC01268 and myristoylated alanine rich protein kinase C substrate(MARCKS),the PI3K/Akt signaling pathway,and the epithelial-mesenchymal transition(EMT)process in GC was demonstrated by western blot analysis.RESULTS The expression of LINC01268 was increased in GC tissues and cell lines.The expression level of LINC01268 was significantly correlated with lymph node metastasis,TNM stage,and tumor differentiation in patients with GC.Over-expression of LINC01268 indicated a poor prognosis for patients with GC,and it had a certain auxiliary diagnostic value for GC.In vitro functional experiments proved that the abnormal expression of LINC01268 further activated the PI3K/Akt signaling pathway and promoted EMT by targeting and regulating MARCKS and ultimately promoted the invasion and metastasis of GC.CONCLUSION This study elucidates that LINC01268 in GC may be an oncogene that further activates the PI3K/Akt signaling pathway and EMT by targeting and regulating MARCKS,and ultimately promotes the invasion and metastasis of GC.LINC01268 may be a potential effective target for the treatment of GC.

关 键 词：Gastric cancer Long non-coding RNA LINC01268 Myristoylated alanine rich protein kinase C substrate INVASION Metastasis 

分 类 号：R73-37[医药卫生—肿瘤]