基于UPLC-QTOF-MS的NFC与FC杨梅汁差异性代谢产物分析  

Analysis of Differential Metabolites between NFC and FC Bayberry Juice Based on UPLC-QTOF-MS

作　　者：孙瑞洋 宣晓婷 崔燕 林旭东 邵兴锋[1] 邓文艺 凌建刚 SUN Ruiyang;XUAN Xiaoting;CUI Yan;LIN Xudong;SHAO Xingfeng;DENG Wenyi;LING Jiangang(College of Food and Pharmaceutical Sciences,Ningbo University,Ningbo 315000,China;Institute of Agricultural Products Processing,Ningbo Academy of Agricultural Sciences,Ningbo 315000,China;National Vegetable Processing Technology Research and Development Center,Ningbo 315000,China;Hunan Yipindongfang Biotechnology Co.,Ltd.,Changsha 410000,China)

机构地区：[1]宁波大学食品与药学学院,浙江宁波315000 [2]宁波市农业科学研究院农产品加工研究所,浙江宁波315000 [3]国家蔬菜加工技术研发专业中心,浙江宁波315000 [4]湖南一品东方生物科技有限公司,湖南长沙410000

出　　处：《食品工业科技》2023年第15期275-282,共8页Science and Technology of Food Industry

基　　金：宁波市公益类科技计划项目(2019C10033&2019C10104);国家重点研发计划项目(2017YFD0400703)。

摘　　要：为探究浓缩还原(From concentrate,FC)和非浓缩还原(Not from concentrate,NFC)杨梅汁成分的差异,本文借助超高效液相色谱-四极杆飞行时间质谱的代谢组学技术分析二者的代谢产物。通过正交偏最小二乘-判别分析实现了对NFC和FC杨梅汁的区分,根据变量投影重要度和差异倍数筛选出9个差异较大的代谢物,经对比发现,FC杨梅汁中对磺基苯甲酸、5-甲基四氢叶酸、矢车菊素、西瑞香素、花椒毒酚和异黄蝶呤等抗氧化活性成分的含量相对较低,而苦味物质(新橙皮苷、橙皮苷和槲皮素-3-O-新橙皮苷)含量相对较高。通过对差异代谢物的代谢途径分析,筛选出5条差异较大的代谢通路,其中苯丙氨酸代谢、黄酮和黄酮醇的生物合成是NFC与FC杨梅汁中差异代谢物的关键代谢途径。本研究揭示了NFC与FC杨梅汁代谢产物的差异性,为NFC杨梅汁的加工与鉴别提供理论参考。This study analyzed the differential composition between from concentrate(FC)and not from concentrate(NFC)bayberry juices by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS).NFC and FC bayberry juice were distinguished from each other by orthogonal partial least squares discriminant analysis,and 9 compounds that significantly differed in abundance between them were selected and identified according to the variable importance in the projection and the fold-change.By comparison,the content of antioxidant active components such as 4-sulfobenzoate,5-methyltetrahydrofolic acid,cyanidin,daphnoretin,xanthotoxol and isoxanthopterin in FC bayberry juice were relatively low,whereas the bitter substances(neohesperidin,hesperidin and quercetin-3-O-neohesperidin)were relatively high.Metabolic pathways were analyzed on account of differential metabolites and five metabolic pathways with great differences were identified,among which phenylalanine metabolism,biosynthesis of flavonoids and flavonols were the key metabolic pathways of different metabolites in NFC and FC bayberry juice.This study revealed the difference of metabolites between NFC and FC bayberry juice,which provided theoretical reference for the processing and identification of NFC bayberry juice.

关 键 词：非浓缩还原杨梅汁 超高效液相色谱-四级杆飞行时间质谱 代谢组学 代谢通路 

分 类 号：TS255.44[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]