茶树CsTMFs的克隆与表达分析  

Cloning and Expression Analysis of CsTMFs Gene in Tea Plant

作　　者：孙明慧 吴琼 刘丹丹 焦小雨 王文杰 SUN Ming-hui;WU Qiong;LIU Dan-dan;JIAO Xiao-yu;WANG Wen-jie(Tea Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230001)

机构地区：[1]安徽省农业科学院茶叶研究所,合肥230001

出　　处：《生物技术通报》2023年第7期151-159,共9页Biotechnology Bulletin

基　　金：安徽省重大专项(202003a06020021);安徽省茶叶产业技术体系(AHCYJSTX-11);安徽省农业科学院成果推广项目(2023YL031)。

摘　　要：基于茶树全基因组数据,筛选鉴定茶树(Camellia sinensis(L.)O.Ktze.)TATA元件调控因子(TATA element modulatory factor,TMF)CsTMFs家族成员。克隆茶树叶片中CsTMFs编码区序列(coding sequence,CDS)全长。使用生物信息学方法对CsTMFs的保守结构域、基因结构、理化性质、蛋白质二级结构和系统发育关系进行分析。基于转录组数据进行组织特异性表达的分析,利用实时荧光定量聚合酶链式反应(real-time fluorescent quantitative PCR,qPCR)方法检测冷胁迫和干旱胁迫下CsTMFs在茶树叶片中的表达情况。结果表明,CsTMFs家族有2个CsTMF基因,序列CDS区长度分别为2934和2904 bp,均具有TMF-DNA-bd和TMF-TATA-bd完整保守结构域。CsTMF1和CsTMF2具有组织表达特异性,CsTMF1在花与茎中表达较高,CsTMF2在成熟叶与茎中表达较高。CsTMF1受冷胁迫诱导,CsTMF2受冷胁迫和干旱胁迫抑制。Based on the whole genome data of tea plant(Camellia sinensis),members of tea plant TATA element modulatory factor(CsTMF)gene family were screened and identified.The full-length coding sequence(CDS)of CsTMFs in the tea plant leaves was further cloned.The conserved domain,gene structure,physical and chemical properties,protein secondary structure and phylogenetic relationship of CsTMFs were analyzed by bioinformatics method.Tissue-specific expression was analyzed based on transcriptome data,and qPCR was used to detect the expressions of CsTMFs in the tea plant leaves under cold stress and drought stress.The result showed that there were two genes of CsTMF gene family in the tea plant.The lengths of CDS region in two CsTMF genes were 2934 bp and 2904 bp,respectively.There were complete conserved domains,TMF-DNA-bd and TMF-TATA-bd in both two CsTMF genes.CsTMF1 and CsTMF2 had tissue expression specificity.CsTMF1 was highly expressed in the flowers and stems,while CsTMF2 was highly expressed in the mature leaves and stems.The results of qPCR showed that CsTMF1 was induced by cold stress,while CsTMF2 was inhibited by cold stress and drought stress.

关 键 词：茶树 TMF 基因克隆 表达分析 

分 类 号：S571.1[农业科学—茶叶生产加工]