古代经典九蒸九晒炮制过程中何首乌饮片物质基础与颜色特征的相关性分析  

Correlation Analysis Between Material Basis and Color Characteristics of Polygoni Multiflori Radix Decoction Pieces During Ancient Classical Nine-time Repeating Steaming and Sun-drying Processing

作　　者：于淼 代悦 刘涛涛 肖永庆[1] 李丽[1] YU Miao;DAI Yue;LIU Taotao;XIAO Yongqing;LI Li(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2023年第16期178-187,共10页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(81773903);中国中医科学院科技创新工程项目(CI2021A04205)。

摘　　要：目的:分析古代经典九蒸九晒炮制过程中何首乌饮片主要化学成分及颜色特征的动态变化规律,探讨二者变化的相关性。方法:采用高效液相色谱法(HPLC)测定九蒸九晒炮制过程中何首乌饮片没食子酸、二苯乙烯苷、大黄素-8-O-β-D-葡萄糖苷、大黄素甲醚-8-O-β-D-葡萄糖苷、大黄素、大黄素甲醚等6种成分含量;采用电子眼技术对九蒸九晒炮制过程中何首乌饮片颜色进行客观表征;利用偏最小二乘法(PLS)对主要成分含量与颜色特征进行相关性分析。结果:何首乌饮片九蒸九晒炮制过程中,随着蒸晒次数的增加,没食子酸含量逐渐增加,其余成分含量呈现波动性变化,二苯乙烯苷、大黄素-8-O-β-D-葡萄糖苷、大黄素甲醚-8-O-β-D-葡萄糖苷含量总体呈减少趋势,大黄素、大黄素甲醚含量总体呈增加趋势。电子眼结果显示,何首乌饮片九蒸九晒炮制过程中颜色总体变深变红变蓝。6种主要成分含量和颜色的系统聚类分析(HCA)和主成分分析(PCA)结果均显示,何首乌饮片九蒸九晒炮制过程中一蒸一晒至四蒸四晒聚为一类,五蒸五晒至九蒸九晒聚为一类。何首乌饮片九蒸九晒炮制过程中没食子酸、二苯乙烯苷与颜色指标呈显著相关。结论:古代经典九蒸九晒炮制过程中何首乌饮片物质基础与颜色特征的总体变化具有一定规律,通过颜色与指标成分相关性模型的建立,可以实现对何首乌饮片炮制程度的判断及质量评价。Objective:To analyze the dynamic changes of the main chemical components and color characteristics of Polygoni Multiflori Radix(PMR)during the process of ancient classical nine-time repeating steaming and sun-drying,and to explore the correlation between the changes.Method:High performance liquid chromatography(HPLC)was used to determine the contents of gallic acid,stilbene glycoside,emodin-8-O-β-Dglucoside,physcion-8-O-β-D-glucoside,emodin and physcion in PMR during the process of nine-time repeating steaming and sun-drying,and electronic eye technology was used to objectively characterize the color of PMR during the process.Partial least squares(PLS)was used to analyze the correlation between the content of main components and the color characteristics.Result:During the nine-time repeating steaming and sun-drying process of PMR,the content of gallic acid gradually increased with the increase of steaming and sun-drying times,and the contents of other components showed fluctuating changes,with an overall decreasing trend in the contents of stilbene glycoside,emodin-8-O-β-D-glucoside and physcion-8-O-β-D-glucoside,and an overall increasing trend in the contents of emodin and physcion.The results of electronic eye analysis showed that the color of PMR became dark,red and blue in the process of nine-time repeating steaming and sun-drying.The results of hierarchical cluster analysis(HCA)and principal component analysis(PCA)of the contents of six main components and the colors showed that the samples steamed and sun-dried for one to four times could be grouped into one category,the other five categories of samples could be grouped into another category.Gallic acid and stilbene glycoside were significantly correlated with the color indexes of PMR during the process of nine-time repeating steaming and sun-drying.Conclusion:The general changes of material basis and color characteristics of PMR during the process of ancient classical nine-time repeating steaming and sun-drying have certain rules,and the establishment

关 键 词：何首乌 古代经典 炮制方法 九蒸九晒 物质基础 颜色特征 相关性分析 

分 类 号：R22[医药卫生—中医基础理论] R943.1[医药卫生—中医学] R28[理学—分析化学] O657[理学—化学]